云南眼镜蛇毒因子用于预致敏大鼠同种心脏移植的实验研究  

Effect of Yunnan-cobra venom factor in overcoming acute humoral rejection after allograft cardiac transplantation in presensitized recipients: experiment with rats

作　　者：李荣[1] 陈刚[1] 郭晖[1] 王大卫[1] 谢琳[1] 王树森[1] 王婉瑜[2] 熊郁良[2] 陈实[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院器官移植研究院教育部器官移植重点实验室卫生部器官移植重点实验室,武汉430030 [2]中国科学院昆明动物研究所毒素室

出　　处：《中华医学杂志》2006年第21期1460-1463,共4页National Medical Journal of China

基　　金：国家高技术研究发展(863)计划(2003AA205009)资助项目;高等学校博士学科点专项科研基金(20040487077)资助项目

摘　　要：目的观察纯化的云南眼镜蛇毒因子（Y-CVF）对预致敏大鼠同种心脏移植急性体液排斥反应的作用。方法BN大鼠到Lewis大鼠连续3次皮肤移植预致敏后行颈部异位心脏移植。将15对大鼠用随机数字法分成2组，实验组（n=8）于心脏移植前24h静脉给予Y-CVF80μg／kg；对照组（n=7）不用Y-CVF。观察移植心的生存时间，移植心停跳后病理学检查排斥类型，免疫组织化学染色观察移植心IgG和补体C3的沉积。结果Lewis大鼠预致敏后抗BN大鼠抗体滴度由0升高至1：1028～1：2056。对照组移植心存活时间为12．71h±13．94h，实验组移植心存活时间为99．50h±38．72h，与对照组比较差异有统计学意义（t=5．599，P〈0．01）。病理检查结果证实，实验组均未发生急性体液排斥，仅见以大量单核淋巴细胞浸润为特征的急性细胞排斥反应。对照组则见以小血管内血栓形成为特征的急性体液排斥反应。免疫组织化学IgG染色实验组和对照组均为阳性，C3染色对照组为阳性，而实验组为阴性。结论使用Y-CVF可克服预致敏大鼠同种心脏移植急性体液排斥反应的发生。Objective To investigate the effect of Yunnan-cobra venom factor （Y-CVF） in overcoming acute humoral rejection after allograft cardiac transplantation in presensitized recipients. Methods Fifteen Lewis rats received the transplantation of full-thickness skin graft of BN rats three times so as to be presensitized. Fifteen pairs of Lewis rat, as recipients of heart, and BN rat, as heart donors, were randomly divided into 2 groups ： experimental group （ n = 8 ）, and control group （ n = 7 ）. The Lewis rats in the experimental group received heart transplantation of the heart of the BN rat 7 - 10 days after the third skin transplantation, and were injected with Y-CVF 80 μg/kg 24 hours before the heart transplantation. The Lewis rat in the control group received only the heart transplantation without Y-CVF injection. Blood samples were collected from all rats before pre-sensitization and 7 days after the third skin transplantation so as to determine the titer of anti-BN rat lymphocyte antibody. 0 and 24 hours, and 6 and 8 days after Y-CVF injection blood samples were collected from the Lewis rats to determine the total complement activity with the complement activity before Y-CVF injection defined as 100%. The survival time of the transplanted heart was observed. After the transplanted hearts stopped to beat, they were resected and underwent HE staining and microscopy. Immunohistochemistry was used to examine the deposition of IgG and complement 3 （ C3 ）. Results The titer of anti-BN rat lymphocyte antibody was 0 before the pre-sensitization, and increased to 1 ： 1028 - 1：2056 7 days after the third skin pre-sensitization. The serum total complement activity of the Lewis rats decreased to 0 twenty-four hours after the Y-CVF injection, recovered to 2.01% - 15.41% 6 days after, and returned to the normal level （89.61% - 109.46% ） 8 days after. The mean survival time of the transplanted hearts of the control group was 12.71 - 13.94 hours （with a range of 1.5 -15 hours）, significantly shorter

关 键 词：眼镜蛇毒素 心脏移植 移植排斥 

分 类 号：R654.2[医药卫生—外科学] R996.3[医药卫生—临床医学]