大鼠脑挫伤后脑组织β-APP表达的研究  

A study on β-APP expression in experimental brain contusion in rats

作　　者：何光龙[1] 贾冬梅[1] 周亦武[1] 孙来晶[1] 邓伟年[1] 戴甲培[2] 刘良[1] 徐小虎[3] 

机构地区：[1]华中科技大学同济医学院法医学系,湖北武汉430030 [2]华中科技大学同济医学院神经生物学教研室,湖北武汉430030 [3]汕头大学医学院法医学教研室,广东汕头515031

出　　处：《法律与医学杂志》2006年第2期115-118,共4页Journal of Law & Medicine

基　　金：国家自然科学基金(30570629);中国博士后科学研究基金(2003034459);湖北省自然科学基金(2003ABA140)

摘　　要：目的观察大鼠实验性脑挫伤后CNS内β-APP蛋白表达时空性规律。方法采用自由落体撞击法致大鼠脑挫伤模型,用免疫组化技术观察伤后β-APP蛋白在不同时间(0.5h、2h、6h、12h、24h、3d、7d、14d)的表达情况,并用计算机图像分析技术作定量统计分析。结果(1)皮质内β-APP蛋白在伤后表达呈一定波动性,0.5h表达明显增加后,逐渐下降,12h降至最低,低于对照组,随后再次表达增加,3d达高峰;(2)伤后24h海马区β-APP表达开始逐渐下降,3d降至最低,14d回到对照组水平;(3)齿状回(DG)内β-APP蛋白在伤后12h低于对照组。结论实验性脑挫伤后皮质β-APP表达时序性规律可为脑损伤时间推断提供一个新的参考指标,但海马区及DGβ-APP变化规律在脑损伤时间推断方面价值不大。Objective To Study the change of β-APP expression in different wound age and site in the experimental traumatic brain injury in rats. Methods The animal model of cerebral contusion was established by impact to the parietal lobe with a free fall weight,β-APP expression in different wound age was observed with Immunohistochemistry. The results were measured quantitatively with computer imaging analysis system. Male Spragure-Dawley rats were divided into 3 groups： normal control, sham operation control and injury group. Results （1）The number of β-APP-positive neurons definitely increase in 0.5 h after brain injury, decline gradually and reach valley bottom in 12h, increase again and reach the peak in 3 d. （2）In hippocampus the number of β- APP-positive neurons definitely increases after 24 h after brain injury, then decline gradually and reach valley bottom in 3d, return to the level of control at 14 d. （3）In dentate gyms （DG） the number of β-APP-positive neurons at 12 h is lower than the control after brain injury.The intensity of the immunoreactivity of β-APP protein changes with time after traumatic brain injury, which might be used for determing injury time, however, the change in hippocampus and DG has little value in determing injury time.

关 键 词：脑挫伤 Β淀粉样前体蛋白 损伤时间 免疫组织化学 法医病理学 

分 类 号：D919.1[医药卫生—法医学]