超排卵周期卵巢的反应性与卵泡颗粒细胞中卵泡刺激素受体表达的关系  

Ovarian response to gonadotropin stimulation depends on the level of follicle stimulating hormone receptor on granulosa cells

作　　者：蔡婕[1] 高惠娟[1] 黄荷凤[1] 

机构地区：[1]浙江大学医学院附属妇产科医院生殖内分泌科,杭州310006

出　　处：《中华妇产科杂志》2006年第6期372-374,共3页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的探讨超排卵周期卵巢的反应性与颗粒细胞中卵泡刺激素受体(FSHR)表达的关系。方法采用蛋白印迹法,测定因输卵管性不孕或者男性因素不孕的60例不孕症患者的卵泡颗粒细胞FSHR蛋白的水平;采用电化学发光法测定血清雌二醇峰值。根据超排卵周期中发育卵泡数,将60例患者分为卵巢低反应组(20例)、卵巢中反应组(20例)及卵巢高反应组(20例),比较各组FSHR蛋白表达水平,并分析FSHR表达水平与卵泡数、雌二醇峰值的相关性。结果(1)卵巢低反应组、卵巢中反应组及卵巢高反应组的FSHR蛋白表达分别为0·19±0·07、0·34±0·16及0·45±0·18,3组比较,差异均有统计学意义(P<0·001);卵泡数分别为(2·5±0·5)、(7·9±1·9)及(21·6±3·8)个,3组比较,差异均有统计学意义(P<0·01);雌二醇峰值分别为3441、7864及22486pmol/L,3组比较,差异有统计学意义(P<0·01)。(2)3组患者超排卵周期颗粒细胞FSHR表达水平与卵泡数及雌二醇峰值均呈显著正相关关系(rs=0·52及0·71,P<0·01)。结论超排卵周期中,卵巢的反应性与颗粒细胞FSHR蛋白表达有关,FSHR表达水平的高低反映不同的卵巢反应类型。Objective To investigate the role of follicle stimulating hormone receptor （FSHR） expression on granulosa cells in ovarian response of women undergoing gonadotropin stimulation. Methods A total of 60 women were recruited and divided into poor, moderate and high responders according to the number of follicles. FSHR expression was detemined by Western blot on granulosa cells obtained by follicular aspiration. The peak levels of serum estradiol （E2 ） were meassured by immunofluorescent assay. Results （ 1 ） The expression of FSHR was significantly different among the 3 groups, being 0. 19 ± 0. 07, 0. 34 ± 0. 16, and 0. 45 ± 0. 18 for poor, moderate and high responders, respectively（ P 〈 0. 001 ）. Poor responders had the lowest peak levels of serum E2 and the least number of follicles. （2） The FSHR was positively correlated with peak level of E2 in serum and the number of follicles （ P 〈 0. 01 for both）. Conclusion Ovarian response to gonadotropin stimulation depends on the different levels of FSHR expression on granulosa cells.

关 键 词：受体 FSH 超排卵 不育 

分 类 号：R714.8[医药卫生—妇产科学]