一个新qnr基因亚型的克隆表达及其多重耐药机制的研究  

Expression of a new qnr gene subtype and mechanism of multidrug resistance

作　　者：李涛[1] 熊自忠[2] 徐元宏[1] 

机构地区：[1]安徽医科大学第一附属医院检验科,合肥230022 [2]安徽医科大学第一附属医院传染病科,合肥230022

出　　处：《中华检验医学杂志》2006年第6期546-549,共4页Chinese Journal of Laboratory Medicine

基　　金：安徽省卫生厅自然科研基金(2002A012);安徽省高等学校自然科学研究项目基金(2005KJ254);安徽省自然科学基金(050430804)

摘　　要：目的研究1株临床分离产酸克雷伯菌中qnr基因新亚型的生物学特性及其多重耐药机制。方法克隆表达qnr新亚型基因，并用聚合酶链反应（PCR）检测β内酰胺酶基因和Ⅰ类整合酶基因的存在情况。结果qnr基因转化子对常见氟喹诺酮类抗菌药物的敏感性较受体菌下降3—25倍，但耐药水平低于临床分离菌株4—256倍。在产酸克雷伯菌中同时检出KLUC-1型超广谱β内酰胺酶（ESBLs）、TEM-1型和OXA-30型广谱β内酰胺酶基因。结论qnr基因可以轻度增加氟喹诺酮类药物的耐药性。携带qnr基因的临床菌株同时携带多种耐药基因。Objective To study the biologic characteristics of the new qnr gene subtype and multidrug resistant mechanism in a clinical isolate of Klebsiella oxytoca. Methods We cloned and expressed the qnr gene, β-1actamase and integrase genes were detected by PCR. Results Susceptibility of transformant containing qnr gene against common fluoroquinolones was 3 - 25 times lower than recipient stain, but drugresistance was 4 - 256 times lower than the clinical isolate. KLUC-1, TEM-1 and OXA-30 genes were also found in the isolate. Conclusions qnr gene can raise the drug-resistance to fluoroquinolones slightly. There are multiple drug-resistant genes in the strain containing the qnr gene.

关 键 词：基因 喹诺酮类 克隆 分子 多药耐药相关蛋白质类 

分 类 号：R516[医药卫生—内科学]