Identification of a Differentially Expressed Gene PPP1CB between Porcine Longissimus dorsi of Meishan and Large White×Meishan Hybrids  

作　　者：Tao HUANG Yuan-Zhu XIONG Ming-Gang LEI De-Quan XU Chang-Yan DENG 

机构地区：[1]Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, Huazhong Agriculture University, Wuhan 430070, China

出　　处：《Acta Biochimica et Biophysica Sinica》2006年第7期450-456,共7页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the National Natural Science Foundation of China(No.30400313);the National High Technology Research;Development Program of China;the Major State Basic Research Development Program of China

摘　　要：To study the molecular basis of heterosis, suppression subtractive hybridization was used to investigate the differences in gene expression between porcine Longissimus dorsi of F1 hybrids Large WhitexMeishan and their female parents Meishan. From two specific subtractive cDNA libraries, the clone.s selected by reverse Northern high-density blot screening were chosen to clone full-length cDNA by rapid amplification of cDNA ends. An expression-upregulated gene for Meishan skeletal muscle, designated protein, phosphatase 1, catalytic subunit, beta isoform （PPP1CB）, was identified. Porcine PPP1CB contains an open reading frame encoding 327 amino acid residues with 13 and 1763 nucleotides in the 5＇ and 3＇ untranslated regions, respectively. A DNA fragment of 721 nucleotides was amplified and a mutation that creates/disrupts a restriction site for endonuclease RsaI was found. The derived amino acid sequence of PPP1CB has high homology with the PPP1CB of three species, Mus musculus （99%）, human （99%） and mouse （100%）. The tissue expression analysis indicated that the swine PPP1CB gene is generally expressed in most tissues. The possible role of PPPICB and its relation to porcine heterosis are discussed.To study the molecular basis of heterosis, suppression subtractive hybridization was used to investigate the differences in gene expression between porcine Longissimus dorsi of F1 hybrids Large WhitexMeishan and their female parents Meishan. From two specific subtractive cDNA libraries, the clone.s selected by reverse Northern high-density blot screening were chosen to clone full-length cDNA by rapid amplification of cDNA ends. An expression-upregulated gene for Meishan skeletal muscle, designated protein, phosphatase 1, catalytic subunit, beta isoform （PPP1CB）, was identified. Porcine PPP1CB contains an open reading frame encoding 327 amino acid residues with 13 and 1763 nucleotides in the 5＇ and 3＇ untranslated regions, respectively. A DNA fragment of 721 nucleotides was amplified and a mutation that creates/disrupts a restriction site for endonuclease RsaI was found. The derived amino acid sequence of PPP1CB has high homology with the PPP1CB of three species, Mus musculus （99%）, human （99%） and mouse （100%）. The tissue expression analysis indicated that the swine PPP1CB gene is generally expressed in most tissues. The possible role of PPPICB and its relation to porcine heterosis are discussed.

关 键 词：differential gene expression PIG HETEROSIS PPP1CB suppression subtractive hybridization 

分 类 号：Q953[生物学—动物学]