致死性高浓度氧对新生鼠发育和肺损伤的影响  

Effect of lethal hyperoxia on pulmonary development and lung injury in neonatal rats

作　　者：朱翠平[1] 杜江[1] 李秋平[1] 封志纯[1] 

机构地区：[1]南方医科大学珠江医院儿科,广东广州510282

出　　处：《南方医科大学学报》2006年第7期945-948,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30571967)~~

摘　　要：目的探讨致死浓度氧(95%)与新生鼠肺发育及损伤的关系,建立支气管肺发育不良动物模型。方法将3dSD新生鼠随机分为高氧(95%)和空气氧组,并和成年鼠对照,观察记录体质量、身长;HE染色观察肺组织形态结构,做辐射状肺泡计数(RAC)。结果(1)高氧新生鼠和成年鼠死亡率分别为12.5%、35.2%,与正常空气氧组比较,新生鼠体质量(18.02±0.68vs13.24±0.59)和身长(8.83±0.25vs6.76±0.51)增长明显减少(P<0.05)。(2)高氧新生鼠RAC值为9.50±1.05,较正常同日龄新生鼠(13.00±1.79)明显减少(P<0.05),高氧成年鼠RAC值(12.67±2.25)比高氧新生鼠为高(P<0.05),跟正常空气组新生鼠差异不明显(P>0.05)。(3)10d新生鼠肺结构已接近成年鼠肺,高氧组新生大鼠可见肺泡壁较薄、结构简单化、数目明显减少,肺泡大小不均,有些肺泡融合、体积增加,部分间隔细胞增多,小血管扩张、充血;成年鼠高氧组肺间质水肿、肺间隔增厚、肺泡腔缩小,肺间隔伊红显色强度增强,肺泡腔内出现红细胞、巨噬细胞及脱落的肺上皮细胞。结论新生大鼠暴露于92%￣95%氧7d可致生长障碍和肺泡化阻滞,出现类似早产儿支气管肺发育不良的肺组织形态特征。Objective To,study the effect of oxygen at lethal levels （95%） on pulmonary development and lung injury in neonatal rats and establish rat models of bronchopulmonary dysplasia. Methods Three-day-old and adult SD rats were assigned to experimental or control groups and subjected to 95%O2 exposure and room air for 7 days. Body weight and length of the rats were recorded, and histological study of the lung tissue and radical alveoli count （RAC） were carried out. Results The mortality rate of the neonatal and adult rats was 12.5% and 35.2% in hyperoxia group, respectively. The newborn rats in hyperoxic group had lower body weight （18.02±0.68 vs 13.24±0.59 g） and length （8.83±0.25 vs 6.76±0.51 cm） than those in the control group （P〈0.05）, with also lower RAC （9.50±1.05 vs 13.00±1.79, P〈0.05）; RAC of the adult rats with hypemxic exposure （12.67±9.25） was higher that of exposed neonatal rats, but not significantly different from that of the adult or neonatal rats in the control group （P〉0.05）. Structure configuration of the rats on the first 10 days of life resembled that of adulthood. The lung of hyperoxic neonatal rats showed thinner walls of alveoli, simple alveolar structure, fewer and larger alveoli, expanded and shrunk alveoli, while the lung of the adult rats displayed thicker septa, smaller space of alveoli, and cells in the space of the alveoli. Conclusion Exposure of neonatal rats to 95%O2 may result in mild pulmonary inflammation in addition to growth impediment and impaired lung development, which shares morphologic similarities to human bmnchopulmonary dysplasia.

关 键 词：高浓度氧 肺损伤 发育 肺泡化 

分 类 号：R722.1[医药卫生—儿科]