不同运动强度与骨骼肌细胞凋亡的时序性实验研究  

Exercises at different intensities and sequential changes of rat skeletal muscle cell apoptosis

作　　者：蔡保塔[1] 余斌[2] 赵江萍 张幸[2] 

机构地区：[1]南方医科大学珠江医院骨科,广东广州510282 [2]南方医科大学南方医院骨科,广东广州510515

出　　处：《南方医科大学学报》2006年第7期1017-1019,共3页Journal of Southern Medical University

摘　　要：目的研究不同运动强度、运动持续时间与骨骼肌细胞凋亡时序性关系,探讨运动与骨骼肌细胞凋亡的关系。方法采用不同速度跑台训练,以力竭运动训练建立骨骼肌运动损伤动物模型,PI染色、流式细胞术检测骨骼肌细胞凋亡率。结果正常运动量组腓肠肌细胞凋亡率在运动第1、3、7、14天之间差异无显著性意义(P>0.05);中等运动量组和力竭运动组腓肠肌细胞凋亡率在运动第1、3、7、14天之间差异都有显著性意义(P<0.05)。安静组与正常运动量组腓肠肌细胞凋亡率无显著性差异;正常运动量组骨骼肌细胞凋亡率变化不明显;中等运动量组、力竭运动组腓肠肌细胞凋亡率在运动训练前3d迅速升高,随后相对稳定;力竭运动组腓肠肌细胞凋亡率最高。结论运动可引起细胞凋亡和加速机体对凋亡细胞清除两种结果,这两种结果之间调控失衡,可能是造成骨骼肌运动损伤原因之一,说明运动具有双向性。Objective To study the effect of exercises of different intensities on rat skeletal muscle cell apoptosis. Methods Rat models of exhaustive exercise-induced skeletal muscle injury was established using tread mill exercises at different speeds, and the skeletal muscle cell apoptosis was detected using propidium iodide （PI） staining and flow cytometry. Result No obvious gastrocnemius cell apoptosis was observed in rats with normal exercise （P〉0.05）, but the cell apoptosis index was statistically significant in moderate and exhaustive exercise groups （P〈0.05）, reaching the highest level in exhaustive exercise group after exercising. The gastrocnemius cell apoptosis index increased obviously on days 1 and 3 of exercise, and stabilized in moderate and exhaustive exercise groups. Conclusion Exercises can evoke cell apoptosis and accelerate apoptotic cell clearance, and the imbalance between the two events during exercises may contribute to skeletal muscle injurv.

关 键 词：凋亡 骨骼肌 跑台训练 流式细胞术 

分 类 号：R87[医药卫生—运动医学]