溴氰菊酯对大鼠脑组织线粒体膜电位和膜流动性的影响  

Effects of Deltamethrin on Mitochondrial Membrane Potential and Membrane Fluidity in Rats Brain Tissue

作　　者：陈丹[1] 石年[1] 李煌元[1] 刘琳琳[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院卫生毒理学系环境与健康教育部重点实验室,湖北武汉430030

出　　处：《癌变．畸变．突变》2006年第4期257-260,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金项目(No.30371225)

摘　　要：背景与目的:研究溴氰菊酯(Deltamethrin,DM)对大鼠脑组织线粒体膜电位及膜流动性的影响。材料与方法:成年雄性Wistar大鼠一次性腹腔注射12.5mg/kg体重DM(溶剂为色拉油),5h、24h、48h、72h后处死,提取皮层和海马的线粒体,分别测定线粒体膜电位、膜流动性、Na+-K+、Ca2+-Mg2+-ATP酶和琥珀酸脱氢酶活力。同时设立对照组,只注射色拉油0.5ml/100g,5h后处死大鼠。结果:大鼠经DM处理后,5h、24h、48h、72h组的线粒体膜电位下降,膜流动性降低,Na+-K+、Ca2+-Mg2+-ATP酶和琥珀酸脱氢酶活力受到抑制,与对照组相比,差异均具有统计学意义(P<0.01),并且与染毒后时间成直线相关关系。结论:DM能明显损害大鼠脑组织线粒体功能,继而引起线粒体氧化磷酸化障碍。BACKGROUND ＆ AIM： To study the effects of deltamethrin（DM） on mitochondrial membrane potential and membrane fluidity in rat brain tissue. MATERIAL AND METHODS： Male adult Wistar rats were treated intraperitonealy with 12.5 mg/kg DM. At 5, 24, 48 and 72 h after injection, we extracted the mitochondria of rat brain tissue to measure the membrane potential, membrane fluidity, activities of Na^＋ -K^＋ -ATPase, Ca^2＋ -Mg^2＋ -ATPase and succinic dehydrogenase.At the same time the control group were merely injected intraperitonealy with O. 5 mg/100 g salad oil and then executed after 5 h. RESULTS： After treatment with deltamethrin, the mitochondrial membrane potential decreased, membrane fluidity was reduced, the activities of Na^＋ -K^＋ -ATPase, Ca^2＋ -Mg^2＋ -ATPase and succinic dehydrogenase were inhibited （P 〈 0.01） . Moreover, there were correlations between those indexes and time after treatment： CONCLUSION： DM had obvious effects on inhibiting the mitochondrial function of rat brain tissue, then caused the impairment of oxidative phosphorylation in the mitochondria.

关 键 词：澳氰菊酯 线粒体 膜电位 膜流动性 

分 类 号：R994[医药卫生—毒理学]