溴氰菊酯对大鼠脑白介素-1β表达的影响  

Effects of Deltamethrin on IL_1βin Rat Brain

作　　者：钟玉芳[1] 李煌元[1] 梁骏华[1] 陈丹[1] 刘琳琳[1] 戴中华[1] 孙敏[1] 石年[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院卫生毒理学系华中科技大学公共卫生学院环境与健康教育部重点实验室,湖北武汉430030

出　　处：《癌变．畸变．突变》2006年第4期261-264,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金项目(No.30371225)

摘　　要：背景与目的:观察给予溴氰菊酯(deltamethrin,DM)后大鼠大脑皮层、海马及下丘脑IL-1β的变化,初步探讨DM对神经免疫功能的影响。材料与方法:雄性SD大鼠共65只,其中15只用于RT-PCR,25只用于免疫组化,另25只用于ATPase活力测定,均随机分为1个对照组和4个DM不同作用时间的实验组。实验组中大鼠一次性腹腔注射溴氰菊酯12.5mg/kg,给药后1、6、24和48h分别检测上述3个脑区中IL-1βmRNA表达和蛋白含量的变化,同时以Na+-K+-ATPase为神经毒性指标,测定上述各时间点3个脑区Na+-K+-ATPase活力,探讨DM不同作用时间与IL-1β之间的关系。结果:皮层IL-1β含量在给药后1~6h升高,而IL-1βmRNA表达的增加在24h^48h与对照组相比差异有统计学意义(P<0.01),但未观察到ATPase活力的变化;给药后1h海马IL-1β含量升高,随之在6h时ATPase活力降低,在24h时IL-1βmRNA出现短暂升高和ATPase活力的恢复,48hATPase活力再度降低;下丘脑IL-1βmRNA的表达在给药后1h开始升高,6h时达峰值,IL-1β蛋白含量24h后才升高,ATPase活力仅在1h时一过性降低。结论:在DM对大鼠中枢神经系统不同脑区的毒作用过程中IL-1β可能发挥不同的作用。BACKGROUND ＆ AIM： To observe the effects of deltamethrin（DM） on the neuroimmunological functions of rats by the changes of IL-1β in the cortex, hippocampus and hypothalamus. MATERIAL AND METHODS： 65 male adult SD rats were separated into 3 groups, one group included 15 rats for RT-PCR, the second group included 25 rats for immunohistochemistry, and the third included 25 rats for detecting the activities of ATPase. Each group was separated randomly into 5 sub-groups including 1 control and 4 DM treated. The DM treated rats received intraperitonea injection of 12.5 mg/kg DM. At 1, 6, 24 and 48 h after injection, we used RT-PCR and immunohistochemistry to characterize the changes of IL-1β mRNA expression and the protein content in different encephalic regions. Then we detected the activities of Na^＋ -K^＋ -ATPase as biomarker to explore the relationship between IL-1β and neural toxicity of DM.RESULTS： From 1 h to 6 h after DM administration, the IL-1β protein content in cortex increased while the gene expression of IL-1β rose after 24 h to 48 h, without neural toxicity in cortex. In the hippocampus, after IL-1β protein increased at 1 h with transient increase of IL-1β mRNA. The activity of Na^＋ -K^ -ATPase decreased at 6 h, and recovered at 24 h, but decreased again at 48 h. The IL-1β mRNA of hypothalamus increased rapidly in 1 h, reaching maximum at 6 h, whilst the IL-1β protein remained higher than that of the control group until 24 h. The activity of Na^＋ -K^＋ -ATPase only showed an apparent reduction at 1 h after injection. CONCLUSION： IL-1β may play different roles in above-mentioned cerebral regions in mediating the neural toxicity of DM in rats.

关 键 词：IL-1Β 溴氰菊酯 神经免疫 

分 类 号：R994[医药卫生—毒理学]