结晶型硫化镍诱发细胞恶变过程中端锚聚合酶mRNA的表达  

Expression of Tankyrase mRNA in the Malignant Transformation of Cells Induced by Crystalline Nickel Sulfide

作　　者：王敏[1] 吕嘉春[1] 

机构地区：[1]广州医学院化学致癌研究所,广东广州510182

出　　处：《癌变．畸变．突变》2006年第4期314-315,共2页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金项目(No.30200235)

摘　　要：背景与目的:探讨结晶型硫化镍(NiS)诱发人支气管上皮细胞恶变过程中端锚聚合酶(tankyrase)mRNA的变化。材料与方法:用逆转录-聚合酶链反应(RT-PCR)技术检测细胞tankyrasemRNA的表达。结果:Tankyrase在细胞恶变过程中不同阶段细胞的表达均高于正常细胞。结论:结晶型NiS诱发人支气管上皮细胞恶性转化涉及了tankyrase活性改变,tankyrase活性改变可能是结晶型NiS诱发肺癌的早期分子事件。BACKGROUND ＆ AIM： To study the expression of tankyrase in human bronchial epithelial malignantly transformed cells induced by crystalline nickel sulfide. MATERIAL AND METHODS： The method of reverse transcription-polymerase chain reaction（RT-PCR） was used to measure the expression of tankyrase mRNA in 16HBE and malignantly transformed cells. RESULTS： The expression of tankyrase in malignantly transformed cells was higher than that in normal cells. CONCLUSION： The overexpression of tankyrase in cell transformation might be related to the carcinogenesis of crystalline nickel sulfide.

关 键 词：结晶型硫化镍 人支气管上皮细胞 转化细胞 成瘤细胞 TANKYRASE 

分 类 号：R994.3[医药卫生—毒理学]