超顺磁氧化铁标记骨髓基质干细胞移植治疗帕金森病鼠的实验研究  

Study on Magnetic Resonance Imaging of SPIO Labeled Bone Marrow Stem Cells in Parkinson’s Rat Model Following Transplantation

作　　者：金莉蓉[1] 洪震[2] 钟春玖[1] 汪洋[3] 

机构地区：[1]复旦大学附属中山医院神经内科 [2]复旦大学附属华山医院神经内科200040 [3]复旦大学上海医学院解剖与组织胚胎学系

出　　处：《中国临床神经科学》2006年第4期348-353,共6页Chinese Journal of Clinical Neurosciences

基　　金：上海市科委资助项目(034119801)

摘　　要：目的:研究应用超顺磁氧化铁(SPIO)标记骨髓基质干细胞(MSCs)移植治疗帕金森病(PD)大鼠后的在体MRI观察。方法:分离、获取大鼠骨髓基质细胞,脂质体转染法将SPIO标记MSCs;制作PD模型,SPIO标记的MSCs移植到PD大鼠右侧纹状体区,应用MRI在体观察脑内移植的骨髓基质细胞的存活和迁徙情况。结果:体外SPIO标记的骨髓基质细胞普鲁蓝染色阳性;脑内移植SPIO标记MSCs的PD大鼠磁共振T2和T2GRE扫描检查显示在移植区呈低信号改变。随时间的延长,移植区信号向周围扩大。脑纹状体区的铁染色也可见SPIO标记MSCs从移植部位向四周迁移。结论:SPIO可用于体外标记MSCs,通过MRI技术可以对标记细胞脑内移植后进行初步的活体示踪,有利于MSCs移植治疗PD后的疗效观察。Aim： To investigate the changes of superparamagnetic iron oxide （SPIO） labeled bone marrow stem cells in magnetic resonance imaging （MRI） in the experimental treatment of Parkinson＇ s disease. Methods： Bone marrow stem cells （MSCs） from adult bone marrow of SD rats were cultured, isolated and purified. Labeled MSCs was achieved with the magnetic resonance contrast agent SPIO, and lipofectamine was used as transfection agent to achieve high transfer efficiencies. MSCs or labeled MSCs were transplanted to the right striatal area of 6- OHDA induced PD rat models. Results： SPIO particles were clearly visible with Prussian blue staining in cells. In vivo tracking magnetic resonance images especially T2＊ gradient echo showed that a dark signal appeared in the transplanted area, the dark signal area became larger as the transplanted times went on. Histochemical staining of brain tissue also showed that SPIO labeled MSCs were moved from the transplanted points to the nearby areas. Conclusion： SPIO particles could effectively label MSCs, and MRI detection of SPIO labeled cells was a promising method and novel approach to analysis the MSCs following transplantation to treat PD.

关 键 词：骨髓基质细胞 超顺磁氧化铁 磁共振成像 帕金森病 移植 

分 类 号：Q813[生物学—生物工程] Q189