丙酮酸盐对牛精液冷冻效果的影响  

Effect of Pyruvate on Cryopreservation of Bull Semen

作　　者：胡传活[1] 杨俊涛[1] 梁琪妹[1] 卢晟盛[1] 韦英明[1] 张明[1] 李广雪[2] 隆有前[2] 卢克焕[1] 

机构地区：[1]广西大学动物繁殖研究所,广西南宁530005 [2]广西大学动物科技学院,广西南宁530005

出　　处：《河南农业科学》2006年第7期105-108,共4页Journal of Henan Agricultural Sciences

基　　金：广西科技攻关项目(桂科攻0235016-4);广西教育厅科研项目(桂教科研〔2002〕136)

摘　　要：在常用的果糖-柠檬酸钠-卵黄-甘油液的基础上,分别添加1.25 mmol/L或2.00mmol/L的丙酮酸钾、丙酮酸钠或丙酮酸钙,以观察丙酮酸盐在牛精液冷冻中的应用效果。冷冻前各组稀释液隔15min分2次加入,之后平衡4h。结果表明,各组精子的冻后活力、复苏率、畸形率和顶体完整率之间没有显著差异,但6个试验组的冻后活力、复苏率和对照组相比有提高趋势。Potassium pyruvate, sodium pyruvate and calcium pyruvate （1.25, 2.00mmol/L respectively） were added to the conventional diluents （the medium containing fructose, sodium citric, egg yolk and glycerol） to test the effects of pyruvates on cryopreservation of bull semen. The extenders were added 2 times in 15minitues interval. Then the diluted semen samples were equilibrated for 4h before frozen. The results showed that there weren＇t significant differences among all the tested samples on progressive motility, recovery rate, deformity and acrosomal integrity, the 6 treated groups tended to demonstrate better progressive motility and recovery rate compared to the control.

关 键 词：丙酮酸盐 牛 精液冷冻 

分 类 号：S823[农业科学—畜牧学]