运动训练诱发大鼠脑梗死周边区域神经突触变化的机制  

The mechanism of synapse changes in the region around a cerebral infarct after mobility training

作　　者：谭来勋[1] 孙圣刚[2] 张双国[1] 

机构地区：[1]武汉大学人民医院神经科,武汉430060 [2]华中科技大学同济医学院附属协和医院神经科

出　　处：《中华物理医学与康复杂志》2006年第7期433-436,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：国家教育部高等学校博士点学科专项科研基金(No.20020487065)

摘　　要：目的研究运动训练对大鼠脑梗死周边区域突触变化的影响及相关机制。方法将150只大鼠制成大脑中动脉闭塞脑梗死模型,并随机分为运动训练组、生理盐水组、阿糖胞苷抑制组、美伐他丁组及对照组。各组大鼠分别于脑梗死7、21及42d时应用免疫组织化学方法和高压液相色谱法观察其脑梗死周边区域胶质纤维酸性蛋白(GFAP)、突触素表达值和胆固醇含量的变化情况。结果运动训练组大鼠脑梗死周边区域GFAP、突触素阳性信号密度值(%)和胆固醇含量均明显高于对照组和阿糖胞苷抑制组(均P＜0．01),而对照组上述指标又显著高于阿糖胞苷抑制组(均P＜0．01)；美伐他丁组突触素表达值及胆固醇含量均低于运动训练组(均P＜0．01)。结论运动训练可促进脑梗死大鼠加强神经突触重建及功能恢复,运动训练致使星形胶质细胞活性增强及刺激胆固醇分泌可能在其中发挥重要作用。Objective To investigate how mobility training affects the synapses and their functioning in the region around a cerebral infarct. Methods One hundred and fifty rats were randomly divided into 5 groups： a motor training group, a normal saline group, an Ara-c inhibition group, a mevastatin group and a control group. Cerebral infarcts were surgically induced in all 150 rats, and the level of either glial fibrillary acidic protein （GFAP） or synaptopbysin, and the cholesterol content around the infarct were observed at 7,21 and 42 days using immunobistochemical staining and high performance liquid chromatography. Results At 7, 21 and 42 days after the infarct model was induced, significant differences in the optical density of either GFAP or synaptopbysin and in cholesterol levels were noted between the motor training group and the control group. Ara-c inhibition was also significantly higher in the controls. The optical density of synaptopbysin and the cholesterol levels were significantly lower in the mevastatin group than in the motor training group. Conclusion Motor skill training can improve synapse redefinition in rats with a model of acute cerebral infarct. Astrocytes may play a crucial role by means of the secretion of cholesterol in the region around the infarct.

关 键 词：突触 胆固醇 星形胶质细胞 运动训练 脑梗死 

分 类 号：R87[医药卫生—运动医学]