绵羊精子提取物对卵母细胞的孤雌激活作用  

Application of sheep sperm extract cytosolic injection in oocytes parthenogenetic activation

作　　者：赛务加甫[1] 张立[1] 彭新荣[1] 郭志林[1] 杨丽[1] 李向臣[1] 何宽军[1] 安志兴[1] 张涌[1] 

机构地区：[1]西北农林科技大学动物科技学院生物工程研究所

出　　处：《西北农林科技大学学报（自然科学版）》2006年第7期9-12,16,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家"863"高技术研究发展计划项目(2001AA213081)

摘　　要：研究了绵羊精子提取物对绵羊卵母细胞的孤雌激活作用，确定了绵羊精子提取物的最佳注射剂量，并将其孤雌激活效果与离子霉素联合6-DMAP法的激活效果进行了比较。结果表明，绵羊精子提取物对绵羊卵母细胞有孤雌激活作用，最佳注射剂量为每个绵羊卵母细胞注入2～4pL浓度5～6mg／mL的绵羊精子提取物；绵羊精子提取物的孤雌激活效果与离子霉素联合6-DMAP法的激活效果差异不显著。It was studied that sheep oocytes were parthenogenetic activated by cytosolic injection of sheep sperm extract. Activation effect of different injection doses of sheep sperm extract were compared to find a feasible injection dose. At the same time activation effect by Ionomycin and 6-DMAP was compared with cytosolic injection of sheep sperm extract. The result showed that oocytes could be parthenogeneticly activated by cytosolic injection of sheep sperm extract,and the best feasible injection dose was 2-4 pL for every oocyte when its concentration was 5-6 mg/mL. There was no distinct difference on activation effect between sheep sperm extract activation group and Ion activation group.

关 键 词：绵羊 精子提取物 卵母细胞 孤雌激活 显微注射 

分 类 号：S826[农业科学—畜牧学]