紫米基因与RFLP标记的连锁分析  

Linkage Analysis of RFLP Markers and the Gene for purple Pericarp of Rice

作　　者：庄杰云[1] 杨长登[1] 钱惠荣[1] 赵成章[1] 郑康乐[1] 

机构地区：[1]中国水稻研究所

出　　处：《Acta Genetica Sinica》1996年第5期373-375,共3页

基　　金：国家科委863计划;美国洛克菲勒基金会的资助

摘　　要：选用种皮呈紫黑色的水稻体细胞无性系变异体黑珍米和其种皮呈无色的原始亲本Ｂａｓｍａｔｉ３７０配制组合，同时应用１２１个ＤＮＡ探针检测了黑珍米与Ｂａｓｍａｔｉ３７０之间的ＲＦＬＰ。应用Ｆ２和Ｆ３群体研究了紫色种皮的遗传控制。结果表明，有一个显性主效基因控制着黑珍米和Ｂａｓｍａｔｉ３７０在种皮颜色上的差异。通过多态性ＤＮＡ探针与种皮颜色的共分离分析，发现该基因与水稻第四染色体上的ＤＮＡ标记ＲＧ３２９和ＲＧ２１４连锁，与ＲＧ３２９和ＲＧ２１４的遗传图距分别为１８．９ｃＭ和２６．３ｃＭ。F2 and F3 populations of the cross of a rice somaclonal variant Hei-zhen-mi with purple pericarp and its donor parent Basmati 370 with white pericarp were used to study the genetic control of purple pericarp. A dominant gene was responsible for the difference of pericarp pigmentation between Hei-zhen-mi and Basmati 370.RFLPs between Hei-zhen-mi and Basmati 370 were assayed using 121 DNA probes. By cosegregation analyses of polymorphic probes and the pigmentation of pericarp, a gene for purple pericarp was found to be linked to DNA markers RG329 and RG214 on chromosome 4 of rice with genetic distances of l89cM and 26.3cM to RG329 and RG214, respectively.This research was supported by grants from the Rockefeller Foundation Rice Biotechnology Program and from the 863 program of the State Science and Technology Commission of China.

关 键 词：水稻 紫色种皮 基因定位 RFLP 紫米基因 

分 类 号：S511.032[农业科学—作物学]