丙肝病毒IgM抗体检测方法的初步研究  

Preliminary Study on the Method for Detecting lgMAntibody to Hepatitis C Virus

作　　者：何红霞[1] 洪世雯[1] 貌盼勇[1] 白雁平[1] 

机构地区：[1]解放军302医院病毒研究室

出　　处：《中国病毒学》1996年第3期264-267,共4页Virologica Sinica

摘　　要：选择东燃公司的重组结构区和非结构区抗原建立的抗ＨＣＶ－ＩｇＭ检测方法，简便、快速、特异性强、重复性好、敏感性高。只在丙肝病人组检出而健康献血员均为阴性，与抗ＨＡＶ、ＨＢＶ的ＩｇＭ抗体无交叉反应，且排除了ＲＦ干扰和ＩｇＧ占位引起的假阳性和假阴性，适用于抗ＨＣＶ－ＩｇＭ的临床检测。对２４例丙肝病人的抗ＨＣＶ－ＩｇＭ检测结果显示，急性丙肝病人血清抗ＨＣＶ－ＩｇＭ检出率较高（７５％，６／８），且随ＡＬＴ正常而消失或滴度下降。慢性病人抗ＨＣＶ－ＩｇＭ检出率为５６．３％（９／１６），其中７例ＩｇＭ持续阳性者为慢性活动性丙肝，说明慢性病人抗ＨＣＶ－ＩｇＭ与疾病的活动性密切相关。结果提示抗ＨＣＶ－ＩｇＭ的检测在急性肝炎的诊断及慢性丙肝的预后和转归上具有临床意义。ndirect ELISA for detecting IgM antibody to HCV was established based on recombinantantigens of HCV structural and nonstructural regions.The result showed it is easy, rapid,repro-ducible, specific and sensitive.Asti HCV IgM was positive only in the sera of patients with hepatitis C,and all negative in healthy donors.There were no cross reactions with IgM antihady toHAV and HBV.The false positive and negative caused by RF and specific IgG were removed.Itis suitable for the clinical detection of IgM antibody to HCV.The sera of 24 patients with hepatitisC were assayed for anti HCV lgM.The data indicated that the positive rate is 75i)fj in thepatients with acute hepatitis C.As ALT being nornial,anti HCV IgM became undetectable or itslevel dropped. Aiiti HCV IgM was detected in 9 of 16(56.3%) patients with chronic hepatitis.Among these 9 patients with anti HCV IgM,7 were with abnormal ALT and 2 with norinalAlT. puiti HCV IgM may persist in chronic active patients.The results suggested that AntiHCV lgM is a useful marker in diagnosis of acute hepatitis C, indicatinery the activrty of chronichepatitis C and guiding the antiviral therapy.

关 键 词：丙型肝炎病毒 IGM抗体 ELISA 

分 类 号：R373.21[医药卫生—病原生物学]