表达反义核酶RNA的转基因水稻对矮缩病毒复制和症状的抑制作用  

Inhibition of Replication of Rice Dwarf Virus in Transgenic Rice Plant Expressing Antisense Ribozyme gene

作　　者：杨文定[1] 吴祖建[1] 王苏燕[1] 刘伟平 叶寅[1] 谢联辉 田波[1] 

机构地区：[1]中国科学院微生物研究所,福建农业大学植物病毒研究所

出　　处：《中国病毒学》1996年第3期277-283,共7页Virologica Sinica

基　　金：863高技术

摘　　要：用基因枪法将含有ＲＤＶ第五片段反义核酶序列基因的植物表达载体ｐＲＯＫＩＩ转化水稻幼胚，在Ｇ４１８存在的条件下，约２～３个月可筛选出抗性愈伤，转入分化培养基中培养可分化出幼苗。经Ｓｏｕｔｈｅｒｎ杂交法检测为阳性的水稻幼苗进行抗病性测定显示，转ＲＤＶ反义核酶基因的水稻植株对ＲＤＶ的复制和症状有显著抑制作用。转基因植株发病较轻，并能部分结实，而对照植株则明显矮化且大多不能抽穗。he plant expression vector pROK II containing antisense ribozyme gene of rice dwarf virus(RDV)S5 was transferred to rice immature embryo by microprojectile bombardrnent.The resis-tant calli were obtained in the presence of G418. Two or three months later these calli could differ-entiate to shoot after transferred to differential medium. The transformation of the regeneratedrice plant was confirmed by Southern blot.Transgenic plants were inocuiated with viruliferous leafhopper(Nephotettix cincticeps)to determine their RDV resistance.The results showed thatthe infection of RDV was not prevented but the symptoms were milder than control.Most of thetransgenic rice plants were fertile while the control was not.In order to assay the RDV replica-tion,virus concentration was detected by ELISA. The results indicated that the repliea.tion of RDVin transgenic plants was obviously inhibited.

关 键 词：水稻矮缩病毒 反义核酶基因 转基因水稻 RNA 

分 类 号：S511.032[农业科学—作物学] S432.41