用套式多聚酶链反应技术监测乙型肝炎病毒的母婴垂直传播  

Detection of Hepatitis B Virus in Perinatal Transmission by Nested Polymerase Chain Reaction

作　　者：黄晓军[1] 黄雅蓉[1] 郑惠童[1] 符玉良[1] 陈火胜[1] 

机构地区：[1]广州市妇婴医院,中山医科大学微生物学教研室

出　　处：《中国病毒学》1996年第4期313-318,共6页Virologica Sinica

基　　金：广州市卫生系统青年科研基金

摘　　要：用套式多聚酶链反应（Ｎｅｓｔｅｄ－ＰＣＲ）技术对１６９对ＨＢｓＡｇ及ＨＢｓＡｇ／ＨＢｅＡｇ阳性孕妇及其新生儿外周血清进行了ＨＢＶ－ＤＮＡ检测。１０３对ＨＢｓＡｇ阳性孕妇及其新生儿外周血清中ＨＢＶ－ＤＮＡ阳性率分别为７２．８％和３３．０％；６６对ＨＢｓＡｇ和ＨＢｅＡｇ双阳性的孕妇及其新生儿外周血清中ＨＢＶ－ＤＮＡ阳性率分别为８６．４％和４３．９％。对５５例ＨＢｓＡｇ及ＨＢｓＡｇ／ＨＢｅＡｇ阳性产妇产后的初乳进行了ＨＢＶ－ＤＮＡ检测，结果ＨＢＶ－ＤＮＡ阳性率为３６．４％。结果表明ＨＢｓＡｇ和ＨＢｅＡｇ双阳性的孕妇及其新生儿外周血清ＨＢＶ－ＤＮＡ检出率较ＨＢｓＡｇ单阳性的孕妇及其新生儿要高，其初乳中ＨＢＶ－ＤＮＡ的检出率也高。还对１０５例注射了乙肝疫苗及高价乙肝特异性免疫球蛋白的６月龄婴儿的外周血清进行了ＨＢＶ－ＤＮＡ检测，结果有２３例阳性。high sensitive method of nested-polymerase chain reaction(Nested-PCR)was estab-lished to detect HBV-DNA in peripheral serum from 169 HBsAg or HBsAg/HBeAg positivepregnant women and their neonates。Positive of HBV-DNA was 72.8% in 103 positive of HB-sAg mothers and 33.0% in their neOnates.In the other 66 both HBsAg and HBeAg positivemothers and their neonates,the HBV-DNA positive was 86.4% and 43.9%,Fifty five samplesof the colostrum from 169 HBsAg or HBsAg/HBeAg positive mothers were detected,the HBV-DNA was found in 20 mothers.These results suggested the e antigen was important in HBV peri-natal transmission.Additional detection was made in 105 cases of 6 months infants from atoveneonates after injection of NBV vaccine and hepatitis B immunoglobulin,23 positive samples werefound in serum。

关 键 词：乙型肝炎病毒 母婴传播 聚合酶链反应 监测 

分 类 号：R373.21[医药卫生—病原生物学] R512.620.1[医药卫生—基础医学]