急性肾功能衰竭犬不同小肠段黏膜超滤量测定及川芎嗪的作用  

Determination of ultrafiltration amount of different small intestinal mucosa in dogs with acute renal failure and the effects of ligustrazine

作　　者：张廉生 彭绪坤 黄德彬[2] 

机构地区：[1]恩施土家族苗族自治州中心医院检验科,湖北省恩施土家族苗族自治州445000 [2]湖北民族学院医学院药理学教研室,湖北省恩施土家族苗族自治州445000

出　　处：《中国临床康复》2006年第27期60-62,共3页Chinese Journal of Clinical Rehabilitation

基　　金：湖北省自然科学基金项目(鄂科技发[2005]28-2005ABA197);湖北省卫生厅重点资助项目(鄂卫发[2003]103-JX1B121);湖北省教育重点资助项目([2004]05-2004D004);湖北民族学院重点资助项目(鄂民院发[2003]15)~~

摘　　要：目的:观察比较单纯透析液及加入不同剂量川芎嗪透析液对急性肾功能衰竭犬不同小肠段黏膜液体吸收能力及超滤量的影响,分析川芎嗪在其中的作用。方法:实验于2004-06/07在湖北民族学院医学院实验中心和恩施自治州中心医院检验科完成。①选用Beagle犬20只,雌性。按随机抽签法分为4组,每组5只:生理盐水组、透析液组、川芎嗪低剂量组和川芎嗪高剂量组。据肠系膜动静脉供血区对犬手术制作隔离空肠袢、空回肠袢和回肠袢,阻流双肾动脉,静脉输入高渗样品液,造成急性肾功能衰竭模型,自输入高渗样品液开始,生理盐水组、对照组、川芎嗪低剂量组和川芎嗪高剂量组前4h内每隔30min分别从各肠袢近端口注入生理盐水、透析液[透析液(mmol/L):甘露醇180,钠60,钾3.7,氯46,碳酸氢盐26.7,钙1.6,葡萄糖35.1,pH7.34]、含川芎嗪(注射液,常州制药厂生产,40mg/支,批号991210)20mg/L透析液、含川芎嗪80mg/L透析液250mL并采各肠袢系膜主干动静脉血各3mL、收集各肠袢液,6h内完成,总时间为360min,各得样品13次。采用生化分析仪器和电解质分析仪器检测血清K+,Na+,Cl-,尿素,肌酐,尿酸浓度。②根据131I标示白蛋白稀释法计算肠袢内透析液容量,计算淋巴吸收量=该时间点该溶质动脉血浆浓度-该时间点该溶质静脉血浆浓度;总吸收量=该时间点该溶质静脉血浆浓度+淋巴吸收量;30min肠袢黏膜平均超滤量=(6h时肠袢内液体容量-总灌入透析液量)/12。③各组间计量资料差异比较用方差分析,两两比较用Fisher’sPLSD检验。结果:犬20只均进入结果分析。①空肠袢液分泌量:实验各时间段各透析组空肠腔内液体分泌量明显高于生理盐水组(P<0.01)。川芎嗪低剂量组排液量在实验30～240min后明显高于其他3组(P<0.01)。②不同小肠段肠袢黏膜液体吸收量:空肠袢总吸收量最弱,而淋巴吸收量最强,分别是其他肠袢的5～8�AIM： To observe and compare the effects of simple dialysate and different dose of ligustrazine dialysate on absorbability and ultrafiltration capacity of intestinal mucosa in dogs with acute renal failure （ARF）, and analyze the effects of the actions of ligustrazine. METHODS： The experiment was conducted in the Department of Clinical Laboratory, Central Hospital Of Enshi Autonomous Prefecture and Experimental Center of Medical College of Hubei College for Nationalities between June and July 2004. ①Twenty female Beagle dogs were selected and randomly divided into 4 groups with 5 animals in each group： normal saline group, dialysate group, low dose ligustrazine group and high dose ligustrazine group. The jejunum, jejunoileum and ileum loop of dogs were isolated through operations according to the arteriovenous region of small intestine mesentery, so as to obstruct both renal arteries. The hypersonic sample solution was input through vein to establish model of ARF. From the beginning of sample-inputting, dogs in normal saline group, control group, low dose and high dose ligustrazine groups were input of normal saline, dialysate [dialysate （mmol/L）： 180 of manicol, 60 of sodium, 3.7 of potassium, 46 of chlorine, 26.7 of bicarbonate, 1.6 of calcium, 35.1 of glucose and 7.34 of pH], dialysate containing 20mg/L ligustrazine and 205 mL of dialysate containing 80 mg/L ligustrazine from the proximate of each ansa interstinalis in the first 4 hours with the interval of 30 minutes. 3 mL of blood was drawn from the bole AtV of each intestinal mucosa, and the collection was accomplished within 6 hours with the total time of 360 minutes, and each sample was obtained for 13 times. The biochemical analytical apparatus and electrolytes analytical apparatus were adopted to detect the concentrations of K^＋,Na^＋,Cl^- in serum as well as urea, creatinine and uric acid. ②According to ^131 I labelled albumen dilution method, the capacity of dialysate inside the ansa interstinalis was calculated. The absorbed do

关 键 词：小肠 超滤 川芎嗪 

分 类 号：R692.5[医药卫生—泌尿科学]