丹参和碱性成纤维细胞生长因子对正加速度重复暴露大鼠脑组织中诱导型一氧化氮合酶mRNA表达的影响(英文)  

Effects of danshen and basic fibroblast growth factor on mRNA expression of inducible nitricoxide synthase mRNA in the brain of rats after repeated +Gz exposure

作　　者：刘红巾[1] 蔡庆[2] 姜建东[2] 占志[2] 朱美财[2] 

机构地区：[1]解放军空军总医院全军航空病研究中心,北京市100036 [2]解放军空军总医院分子生物学研究中心,北京市100036

出　　处：《中国临床康复》2006年第27期156-157,160,共3页Chinese Journal of Clinical Rehabilitation

基　　金：全军医药卫生科研基金课题(98D033);空军后勤部科研基金课题(KH9919076)~~

摘　　要：背景:正加速度引起急性脑功能障碍及其防护是航空医学研究的重要课题之一,但其病理机制尚不清楚,防护措施有待进一步探讨。目的:观察诱导型一氧化氮合酶在正加速度重复暴露大鼠脑组织中的表达变化,分析碱性成纤维细胞生长因子和丹参对反复高正加速度暴露致脑损伤的防护作用。设计:随机对照动物实验。单位:解放军空军总医院分子生物学研究中心。材料:实验于2000-04/08在解放军空军总医院分子生物学研究中心完成。选用健康清洁级SD大鼠20只,按随机数字表法分为5组,即对照组、正加速度暴露组、碱性成纤维细胞生长因子组、丹参组和生理盐水组,每组4只。方法:将所有大鼠固定于动物离心机的转臂上,头朝向离心机轴心。除对照组外,其余各组大鼠正加速度暴露G值为+14Gz,增长率1.5G/s,峰值持续时间45s,共作用3次,两次中间间歇30min。对照组大鼠亦在动物离心机上经历类似实验步骤,所承受的G值为+1Gz。碱性成纤维细胞生长因子组和丹参组大鼠分别于离心前30min和离心后即刻腹腔注射碱性成纤维细胞生长因子(100μg/kg)或丹参注射液(15g/kg),生理盐水组则给予等量生理盐水。于暴露后6h麻醉断头取脑,保存于液氮内用于RNA的提取。用半定量反转录聚合酶链反应方法检测各组大鼠脑组织中诱导型一氧化氮合酶mRNA的表达水平,以相对表达量即诱导型一氧化氮合酶/甘油醛-3-磷酸脱氢酶的比率来计算。主要观察指标:各组大鼠脑组织中诱导型一氧化氮合酶mRNA的表达水平。结果:各组大鼠脑组织中诱导型一氧化氮合酶mRNA的表达水平比较:①正加速度重复暴露组显著高于对照组[0.452±0.014,0.065±0.008(P<0.01)]。②碱性成纤维细胞生长因子组和丹参组显著低于正加速度暴露组[0.196±0.010,0.183±0.011,0.452±0.014(P<0.01)]。结论:正加速度重复暴露可引起大鼠脑内诱导型一氧�BACKGROUND： ＋Gz-induced acute dysencephalia and.its protection is one of the significant topics in Aero-medical researches. Its pathological mechanism, however, is still unclear and protective measures should be developed further. OBJECTIVE： To observe the expression of inducible nitricoxide synthase （iNOS） in brain tissue after ＋Gz exposure and to analyze the protective effects of danshen and basic fibroblast growth factor （bFGF） on repeated ＋Gz exposure-induced brain injury. DESIGN： Randomized controlled animal study. SETTING： Researching Center of Molecular Biology, Air-force General Hospital of Chinese PLA. MATERIALS： The experiment was carried out at the Researching Center of Molecular Biology, Air-force General Hospital of Chinese PLA from April to August 2000. A total of 20 healthy SD rats of clean grade were divided into 5 groups according to randomly digital table, including control group, ＋Gz exposure group, bFGF group, danshen group and saline group with 4 in each group. METHODS： All rats were fixed on rotatory arm of centrifugal apparatus, and their heads were towards core of the apparatus. Except the rats in control group, the value of ＋Gz exposure was ＋14 Gz, and the growth rate was 1.5 G/s. The exposure at peak value lasted for 45 s. ＋Gz exposure was done for three times, and the interval was 30 minutes. Rats in the control group were also treated with the same ＋Gz exposing procedure, but the G value was ＋1 Gz. Rats in bFGF group and danshen group were intrapertoneally injected with 100 μg/kg of bFGF and/or 15 g/kg of danshen solution, respectively, at 30 minutes ＂before centrifugation .and immediateness after eentrifugation; moreover, rats in saline group were injected with the same volume of saline. Six hours after exposure, rats were cut off their heads to obtain the brains which were maintained in liquid nitrogen for RNA extraction. The expression of iNOS raRNA in brain tissues of the rats in each group was detected with semi-quantitative rever

关 键 词：丹参 加速度 一氧化氮合酶 基因表达 成纤维细胞生长因子 碱性 

分 类 号：R856.2[医药卫生—航空、航天与航海医学]