氯化两面针碱对大鼠心肌缺血再灌注损伤的保护作用(英文)  

Protective effects of nitidine chloride on rats during myocardial ischemia/reperfusion

作　　者：韦锦斌[1] 龙盛京[2] 覃少东[1] 黄仁彬[1] 宁宗[3] 潘宇政[4] 王乃平[5] 

机构地区：[1]广西医科大学药理学教研室,广西壮族自治区南宁市530021 [2]广西医科大学化学教研室,广西壮族自治区南宁市530021 [3]广西医科大学第一附属医院急诊科,广西壮族自治区南宁市530021 [4]广西医科大学第一附属医院中医科,广西壮族自治区南宁市530021 [5]广西中医学院药理学教研室,广西壮族自治区南宁市530001

出　　处：《中国临床康复》2006年第27期171-174,共4页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金资助课题(30360118);广西科学基金资助课题(桂科青0640041)~~

摘　　要：背景:研究表明,自由基增多致脂质过氧化是缺血再灌注损伤的主要因素。性味苦辛平,有行气止痛、活血化瘀、祛风通络功用的芸香科植物两面针提取物有抗氧化作用。目的:观察氯化两面针碱对大鼠心肌缺血再灌注损伤的作用,并分析其作用途径。设计:随机对照实验。单位:广西医科大学药理学教研室和化学教研室。材料:选用健康Wistar大鼠60只,雌雄各半,体质量250～300g。氯化两面针碱(广西医科大学化学教研室提供,批号:20050609)。MS4000U生物信号定量记录分析系统(广州市龙飞达科技有限公司)。日立7170A型全自动生化分析仪。722N可见分光光度计(上海精密科学仪器有限公司)。盐酸维拉帕米(上海禾丰制药有限公司,批号:020701,2mL/支)。丙二醛、超氧化物歧化酶测定试剂盒(南京建成生物工程研究所产品)。方法:实验于2004-06/2006-05在广西医科大学药理学教研室和化学教研室完成。①将心电图正常大鼠60只分性别按随机数字表法分为6组:假手术组,模型组,氯化两面针碱2,1,0.5mg/kg3个剂量组,阳性对照组,每组10只,雌雄各半。假手术组:穿线不结扎,90min后结束实验;其余50只大鼠:结扎冠状动脉左前降支缺血30min,再灌注60min。阳性对照组、不同剂量氯化两面针碱组、模型组:在结扎冠状动脉左前降支前10min经股静脉分别注射维拉帕米2mg/kg,氯化两面针碱2,1,0.5mg/kg,5mL/kg,等体积生理盐水。②再灌注的同时用标准肢体Ⅱ导联心电图连续监测,记录60min内各组心律失常类型、发生率及持续时间;并记录再灌注15min及60minST段的变化。③实验完毕,采用全自动生化分析仪检测血清心肌酶学指标。分别用硫代巴比妥酸法与黄嘌呤氧化酶法测定心肌组织丙二醛含量及超氧化物歧化酶活力。④两组间计量和计数资料差异比较采用t检验和χ2检验。主要观察指标:各组大鼠心律失常发生情况BACKGROUND： Studies indicated that lipid peroxidation due to increase of free radical is the key factor of ischemia/reperfusion injury. Shinyleaf pricklyash root extracts, rutaceae plant, is bitter in taste, no stimulation, which has the effects of promoting qi, relieving pain, promoting blood circulation by removing blood stasis, dispelling wind and dredging collaterals and antioxidation. OBJECTIVE： To observe the influence of nitidine chloride on myocardial ischemia/reperfusion injury in rats and analyze its mechanism. DESIGN： Randomized controlled study. SETTING： Department of Pharmacology and Department of Chemistry, Guangxi Medical University. MATERIALS： A total of 60 healthy Wistar rats were selected, half male and half female, with the body mass of 250-300 g. Nitidine chloride was provided by Department of Chemistry, Guangxi Medical University, batch number 20050609. MS4000U biological signal quantitative record analysis system, 722N evident spectrephotometer, hydrochloric acid verapamil （batch number 020701, 2 mL in each）, malondialdehyde （MDA） and superoxide dismutase （SOD） kit were purchased from Guangzhou Longfeida Technology Co., Ltd., Shanghai Precision Scientific Instruments Corporation, Shanghai Harvest Pharmaceutical Co, Ltd. and Nanjing Jiancheng Bioengineering Institute, respectively. Hitachi 7170A full automatic biochemistry analyzer was also applied. METHODS： The experiment was performed at the Department of Pharmacology and Department of Chemistry, Guangxi Medical University between June 2004 and May 2006. ①Totally 60 ＂healthy Wistar rats with normal ECG （half male and haft female） were randomly divided into 6 groups： sham operation group, model group, 2, 1, 0.5 mg/kg nitidine chloride groups, positive control group with 10 rats in each group. The rats in the sham operation group received threading without deligation, and 90 minutes later the experiment was accomplished. Other 50 rats received left anterior descending branch of coronary artery delig

关 键 词：芸香科 心肌再灌注损伤 心律失常 心肌缺血 

分 类 号：R542.2[医药卫生—心血管疾病]