螺旋藻藻胆体核心复合物结构与功能的研究──四种变藻蓝蛋白复合物的分离及光谱特性的研究  

STRUCTURE-FUNCTION RELATIONSHIP AND ENERGY TRANSFER IN ALLOPHYCOCYANIN CORE OF SPIRULIA PLATENSIS──STUDY OF SEPARATION AND SPECTRAL CHARACTERISTICS OF FOUR FORMS OF ALLOPHYCOCYANIN COMPLEXES

作　　者：王慧[1] 赵井泉[1] 张建平[1] 杨紫萱[1] 蒋丽金[1] 

机构地区：[1]中国科学院感光化学所

出　　处：《生物物理学报》1996年第3期394-400,共7页Acta Biophysica Sinica

基　　金：国家自然科学基金

摘　　要：从螺旋藻（Ｓｐｉｒｕｌｉｎａｐｌａｔｅｎｓｉｓ）的藻胆体中分离出四种不同光谱形式的变藻蓝蛋白（ＡＰＣ）复合物：ＡＰⅠ、ＡＰⅡ、ＡＰⅢ、ＡＰＢ，利用吸收光谱、荧光先谱（室温和低温）以及荧光激发偏振光谱比较了ＡＰＣ复合物三聚体和单体的光谱特性。四种ＡＰＣ复合物三聚体的最大吸收和最大发射峰位置各不相同，ＡＰⅡ和ＡＰⅢ位于短波区（最大吸收波长在～６５０ｎｍ．最大发射波长在６６２～６６４ｎｍ），ＡＰⅠ和ＡＰＢ位于长波区（最大吸收波长在～６５５ｎｍ，最大发射波长在～６８０ｎｍ），低温下的荧光发射光谱均有红移。在能量传递中，各种不同形式的ＡＰＣ复合物表现出不同的功能，以实现能量的高效传递：ＡＰⅡ和ＡＰⅢ是能量传递的中介，ＡＰⅠ和ＡＰＢ作为终端发射基因，分别将激发能传给反应中心。通过荧光激发偏振光谱研究了四种ＡＰＣ复合物及其单体内各色团间的相互关系及其在能量传递中的取向和功能。Four different allophycocyanin(APC) complexes were isolated from phycobilisomes (PBS)of Spirulia platensis, and were characterized with respect to absorption spectra, low temperature (-196℃) and room temperature (24℃) fluoascence spectra. Both AP Ⅱ and AP Ⅲ are short-wavelength components with absorption maximum at 650nm and fluorescence emission at 662nm-664nm, while APⅠand APB at long wavelength region show absorption maximum at 665nm, fluoresence emission at 680nm. At low temperature (-196℃),their fluoresrm emission have red shack by 4-5num. This indicates that different APC complexes function different in order to realize energy transfer efficiently, APⅡand APⅢare the excitation energy transfer bridge linking the phycocyanin with APⅠand APB which are the final aerepters in the PBS and transfer the excitation energy to reaction center. The fluorescence polarization spectra were used to study the interaction between different chromophores and their functions and orientations in energy transfer.

关 键 词：藻胆体 螺旋藻 变藻蓝蛋白 复合物 结构 功能 

分 类 号：Q949.220.7[生物学—植物学]