三种因素对紫膜（PM）薄膜中菌紫质（BR）光反应类型的影响  

EFFECTS OF THREE FACTORS ON TYPES OF PHOTOREACTION OF BACTEROPRHODOPSIN (BR) IN PURPLE MEMEBRANE (PM) FILM

作　　者：王敖金[1] 胡坤生[1] 

机构地区：[1]中国科学院生物物理研究所

出　　处：《生物物理学报》1996年第3期499-504,共6页Acta Biophysica Sinica

基　　金：中国科学院"八五"重大项目基金

摘　　要：由中性和碱性ｐＨ成膜液形成的ＰＭ薄膜在不同ＲＨ时ＣＤ语及其不同波长预光照产生的ＤＣＤ谱的研究发现，不同波长预光照产生的ＤＣＤ谱，按照在７００－４６０ｎｍ间的特征可分为全正型、长正短负型和短正长负型三大类。无论成膜液的ｐＨ是中性还是碱性，也不管ＰＭ薄膜的ＲＨ如何，≤４２８ｎｍ的近紫外光照都产生全正型ＤＣＤ谱，而≥６０ｎｍ的光照都产生短正长负型ＤＣＤ谱，但是５０６ｎｍ－５９０ｎｍ间的光照产生的ＤＣＤ谱却不仅与成膜液的ｐＨ有关，而且也与ＰＭ薄膜的ＲＨ有关，成膜液ｐＨ为１０．０的ＰＭ薄膜在ＲＨ７５％时未见有长正短负型ＤＣＤ谱。在近紫外的Ｍ峰区第一、三类ＤＣＤ谱无正峰，而在第二类中为正峰。这些特征都是ＰＭ薄膜中不同结构状态的ＢＲ分子配分浓度分布变化的反映。结果认为，成膜液的ｐＨ、膜所处的ＲＨ和预光照的波长均对ＢＲ的光循环反应有调控作用。这些信息可用于ＢＲ的调控研究，对ＢＲ分子器件的研制开发有重要的参考价值。The CD spectra and DCD spectra, due to pre-illumination with different wavelengths,of purple membrane (PM) films, prepared from neural and alkali film-forming solutions were studied. The results show that, DCD spectra in the range of 700-460nm due to pre-illumination, may be divided into thine types: (1)all positive peaks (2)positive and (3)negative at the longer wavelength side. If the wavelengths of pre - illumination ≤428nm. type 1 occured,≥ 600nm type 3, independence of pH of film-forming solutions and of relative humidity (RH) which the film was in, but between 506-590nm. the, type of DCD was dependent not only on pH of the solutions, but also on RH. Especially, none of type 2 of DCD of the film at RH 75%, from the solution of pH 10.0, was occurred. For the peak (or peaks) from the contribution of M(M peak) in the near ultraviolet, it wu positive in the type 2, hilt nonpositive in the types 1 and 3. These features reflect the changes of the distribution of flactional concentrations of different molecular states of BR. The results suggest that, all of PH of the film-forming solutions, the RH which the film in, and the wavelength which pre-illuminated with, may play an mportaant roles in the regulation and control of photocycle reaction of BR. It is possible to regulate and control the properties of BR. The data in this article are valuble in developing the BR-based molecular devices.

关 键 词：生物膜 紫膜 菌紫质 光反应 

分 类 号：Q734[生物学—分子生物学]