大豆异黄酮的AB-8树脂吸附梯度洗脱纯化精制工艺研究  

Refining Isoflavone by AB - 8 Resin Absorption and Terraced Concentration Desorption

作　　者：王路[1] 崔洪斌[1] 孙丽艳[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院,哈尔滨150086

出　　处：《中国粮油学报》2006年第3期136-139,共4页Journal of the Chinese Cereals and Oils Association

基　　金：国家自然科学基金资助项目(30240073)

摘　　要：以纯化精制大豆异黄酮为目的,研究了以AB-8大孔树脂为吸附剂,不同浓度梯度有机溶剂A 洗脱流程的纯化精制效果。结果表明,以4．5mg大豆异黄酮／mL树脂的上样量,B溶剂→30％A溶剂→ 70％A溶剂一次上柱洗脱,40％A溶剂→70％A溶剂二次上柱洗脱的工艺流程,可以实现较高纯度大豆异黄酮的获得。In order to purify and refine soybean isoflavone, a process of absorption by using macro - pore AB - 8 resin and terraced concentration desorption was developed. The obtained proper process is as follows： isoflavone/ resin of 4.5mg/mL , B solvent→30% A solvent→70% A solvent for the first desorption, and then 40% A solvent→70% A solvent for the second desorption. The produced soybean isoflavone is of high purity.

关 键 词：大豆异黄酮 大孔树脂 纯化 梯度洗脱 

分 类 号：TS214.2[轻工技术与工程—粮食、油脂及植物蛋白工程] O657.72[轻工技术与工程—食品科学与工程]