邻甲氧基桂皮醛对IL-1刺激脑微血管内皮细胞COX活性及PGE_2释放的影响  

Effect of 2-methoxycinnamaldehyde on activity of COX and PGE_2 release in cerebral microvascular endothelial cells stimulated by IL-1

作　　者：郭建友[1] 杨元宵 赵保胜[1] 刘洪斌[1] 李兰芳[1] 马悦颖[1] 郭淑英[1] 霍海如[1] 姜廷良[1] 

机构地区：[1]中国中医科学院中药研究所 [2]浙江中医学院,浙江杭州310053

出　　处：《中国中药杂志》2006年第13期1087-1090,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金重大项目(90209006)

摘　　要：目的:观察桂枝汤有效部位A分离的邻甲氧基桂皮醛对发热相关的环氧酶(COX)和前列腺素E2(PGE2)的影响。方法:建立大鼠脑微血管内皮细胞(rCMEC)培养,进行Ⅷ因子相关抗原鉴定。待细胞生长至融合状态后加入不同含量的邻甲氧基桂皮醛(12.5,25,50,100,200μg.mL-1)孵育3 h,之后以30 ng.mL-1的IL-1刺激12 h。ELISA方法检测细胞培养液中PGE2的含量及细胞内COX-1和COX-2的活性。结果:Ⅷ因子抗体免疫组化染色可见90%以上的培养细胞呈阳性,确认为rCMEC。暴露于30 ng.mL-1IL-1后,rCMEC内COX-2活性及释放的PGE2量显著增加,COX-1活性变化无统计学差异。加入不同含量的邻甲氧基桂皮醛后,随含量增加可下调COX-1,COX-2活性及PGE2量,且呈剂量依赖关系;至含量为200μg.mL-1时,COX-2活性及释放的PGE2与IL-1单独作用组相比均有显著性差异,COX-1活性虽有所降低,但无统计学上的显著差异。结论:邻甲氧基桂皮醛能下调IL-1刺激rCMEC释放升高的PGE2,作用机制可能与抑制COX-2活性有关。Objective： To observe the effect of 2-methoxycinnamaldehyde（isolated from fraction A of Guizhi Tang） on activity of COX and PGE2 release in rat cerebral microvascular endothelial cells （rCMEC） stimulated by IL-1. Method： rCMEC were cultured, and identified by immunohistochemistry for von Willebrand factor （Ⅷ factor, a marker for all endothelial cells） in cyloplasm of the cells. Different concentratiom of 2-methoxycinnamaldehyde were added respectively and incubated for 3 hours, then stimulated for another 12 hans by IL-1. ActivitiES of COX-1 and COX-2 in rCMEC, and production of PGE2 in the conditioned media were measured by ELISA. Result： Positive immunostaining for Ⅷ factor was present diffusely in the cytoplasm of 〉 90% rCMEC. After being exposed to 30 ng·mL%-1 IL, the activity of COX-2 in rCMEC and the production of PGE2 in conditioned media were higher than those of control group, while there was no difference on activity of COX-1 in the two groups. 2-methoxycinnamaldehyde could down-regulate them in concentration-dependently, and significant differences on the activity of COX-2 and amount of PGE2 were showed in 200 μg·mL^-1 concentration. Conclusion： 2-methoxycinnamaldehyde can affect the PGE2 release in rCMEC induced by IL-1, which might be related with its inhibition on the activity of COX-2.

关 键 词：邻甲氧基桂皮醛 脑微血管内皮细胞 白介素1 环氧酶 前列腺素E2 

分 类 号：R285.5[医药卫生—中药学]