枳椇子提取物对实验大鼠肝组织中TIMP-1与MMP-13表达的影响  

Effect of Hovenia dulcis extract on expression of MMP-13 and TIMP-1 in hepatic tissue

作　　者：刘秀玲[1] 张洪[1] 王飞[1] 

机构地区：[1]武汉大学人民医院药学部

出　　处：《中国中药杂志》2006年第13期1097-1100,共4页China Journal of Chinese Materia Medica

基　　金：湖北省科技攻关项目(202AA301C61)

摘　　要：目的:探讨枳子提取物对实验大鼠肝组织基质金属蛋白酶-13(MMP-13)与金属蛋白酶组织抑制因子-1(TIMP-1)表达的影响。方法:SD大鼠48只,随机分为2组:正常对照组(16只)与模型组(32只),用四氯化碳制备大鼠肝纤维化模型,共造模6周,6周末各组分别处死大鼠8只,取肝脏进行HE染色;模型组大鼠随机分为3组:自然恢复组、对照组和给药组,于12周末处死各组大鼠,取肝脏,运用半定量逆转录聚合酶链反应(RT-PCR)检测肝组织中MMP-13 mRNA与TIMP-1 mRNA的表达。结果:MMP-13 mRNA的表达在各组之间并无显著性差异,而TIMP-1mRNA在各组之间有显著性差异,与正常组相比,自然恢复组大鼠肝组织TIMP-1 mRNA显著升高(P<0.05),而与自然恢复组相比,对照组和给药组大鼠肝组织TIMP-1 mRNA的表达显著降低(P<0.05),其中给药组TIMP-1 mRNA比对照组显著降低(P<0.05)。结论:枳子提取物逆转肝纤维化的机制可能是减少肝纤维化大鼠肝脏中TIMP-1mRNA的表达,逐渐恢复肝脏胶原降解系统,从而逆转肝纤维化。Objective： To investigate the effects of Howenia dulcis extract on mRNA expression of MMP-13 and TIMP-1 mRNA in hepatic tissue in experimental rats. Method： 48 male Sprague-Dawley mrs were randomly divided into 2 groups：normal group（16） and model group（32）, hepatic fibrosis was induced by CCI4 for 6 weeks in rats, 8 rats were sacrificed at the end of the 6^th week from every group respectively, HE staining of hepatic tissue was performed; In the model group,rats randomly subdivided into 3 groups：spontaneous recovery group, control group and medication administration group, 8 rats were sacrificed at the end of the 12^th week from every group respectively, the mRNA levels of MMP-13 and TIMP-1 in hepatic tissue were assayed by semi-quantitative RT-PCR. Result： The mBNA expression of MMP-13 among the 4 groups were not statistically significant, but the mBNA expression of TIMP-1 among the 4 groups were statiscally significant. The levels of TIMP- 1 mRNA were significantly increased in control group and medication administration group compared, with those in the model group （ P 〈0.05）, and reverse effects of medication administration groups were significantly high than those of control group （ P 〈0.05）. Conclusion： Inhibition of the mRNA expression of TIMP-1 may be the mechanism of reversing hepatic fibrosis H. dulcis, for thus collogen degradation system was recoveried gradually.

关 键 词：枳棋子 肝纤维化 基质金属蛋白酶-13 金属蛋白酶组织抑制因子-1 

分 类 号：R285.5[医药卫生—中药学]