人重组pcDNA3人钠/碘同向转运体核表达质粒的构建  

Construction of recombinant human eukaryotic expression vector pcDNA3-hNIS

作　　者：张宏飞[1] 严煜[1] 张裕东[1] 

机构地区：[1]南通大学附属医院心胸外科,南通226001

出　　处：《南通大学学报（医学版）》2006年第4期242-243,246,共3页Journal of Nantong University(Medical sciences)

摘　　要：目的:获取、构建并鉴定携带人钠/碘同向转运体(hNIS)基因的pcDNA3.0质粒载体。方法:利用逆转录和聚合酶链反应获取Graves甲亢患者甲状腺NIS基因可编码区片段。与pUCm-T质粒载体连接、经转化、蓝白斑筛选、酶切鉴定,序列测定鉴定,获得pUCm-T-重组质粒。pUCm-T-重组质粒亚克隆得到pcDNA3-hNIS重组质粒。结果:所构建的pcDNA3-hNIS重组质粒中的hNIS基因序列与文献报道序列一致。结论:成功构建pcDNA3-hNIS重组质粒。Objective： To construct and identify recombinant human eukaryotic expression vector with the Sodium/ Iodide symporter （ NIS ） genes. Methods ： Human Sodium/ Iodide symporter genes cDNA were amplified from thyroid tissue of the patient suffering from Graves disease by Rt - PCR , arid cloned into pUCm - T - hNIS , blue/ white color selection and restriction enzyme analysis, pUCm- T- hNIS was sequenced and subcloned into pcDNA3 -hNIS. Results：The results showed that the sequence data of hNIS gene in recombinant plasmid corresponded to those of reference report. Conclusion ：Successfully constructed pcDNA3 - hNIS recombinant plasmid can provide academic evidence for further research.

关 键 词：钠/碘同向转运体 基因转移 基因治疗 逆转录聚合酶链反应 

分 类 号：Q78[生物学—分子生物学]