胶州湾具抗菌活性海洋链霉菌的筛选及菌株M097的代谢产物  

Screening of Marine Streptomyces with Antibacterial Activity from JiaoZhou Bay and Studies on Metabolites from Strain M097

作　　者：崔洪霞[1] 王淑军[2] 李富超[1] 秦松[1] Khaled A Shaaban 

机构地区：[1]中国科学院海洋研究所 [2]淮海工学院,连云港222005 [3]德国哥廷根大学有机生物分子化学系,哥廷根d37077

出　　处：《中国天然药物》2006年第4期308-311,共4页

基　　金：青岛市科技计划项目(05-2-JC-56);江苏省海洋生物技术重点实验室开放课题项目(No.2005HS002)~~

摘　　要：目的:筛选具有抗菌活性的海洋链霉菌并分离鉴定其代谢产物。方法:利用8株受试菌作为抗菌活性筛选模型,以杯碟法对采集自胶州湾海洋的256株链霉菌进行抗菌活性的筛选;对具有抗菌活性的海洋链霉菌M097进行发酵条件的优化,采用乙酸乙酯提取和柱层析等方法,分离发酵液中的代谢产物。结果:具有抗菌活性海洋链霉菌的比例为22%;通过ESI-MS、EI-MS、NMR等波谱数据进行分析,鉴定了3个活性代谢产物的结构。结论:具有抗菌活性的海洋链霉菌的比例很高;海洋链霉菌M097在M+2培养基中产生的3个活性代谢产物均属于大环内酯类抗生素,与Streptomyces griseus产生的feigrisolides A、B和C的结构一致。AIM： To get inhibitory bacteria compounds from marine Streptomyces. METHOD： Using 8 test strains, 256 Streptomyces from ocean were screened with their activities against bacteria. Strain M097 was selected to get an optimum condition. Active compounds have been isolated from the crude extraction by fermentation, ethyl acetate extraction, column chromatography etc. RESULT： The proportion of active marine streptomyces was 22%. 3 compounds produced by marine Streptomyces M097 were identified as feigrisolides A, B and C respectively. CONCLUSION： Marine Streptomycetes showed promising prospects in discovery of inhibitory bacterial substances.

关 键 词：海洋链霉菌 M097 代谢产物 受试菌株 

分 类 号：TQ465.92[化学工程—制药化工]