系统性红斑狼疮患者外周血多形核细胞5-脂氧化酶mRNA的表达研究  

Expression of 5-lipoxygenase mRNA in the peripheral polymorphonucler leukocytes of patients with systemic lupus erythematosus

作　　者：朱远胜 彭学标[2] 

机构地区：[1]江西省龙南县人民医院皮肤科,江西龙南341700 [2]南方医科大学附属南方医院皮肤科,广东广州510515

出　　处：《赣南医学院学报》2006年第3期335-336,共2页JOURNAL OF GANNAN MEDICAL UNIVERSITY

摘　　要：目的:探讨5-脂氧化酶(5-LO)在系统性红斑狼疮(SLE)患者外周血多形核细胞(PMNS)中的表达水平,及其在SLE发病机制中的作用及意义。方法:应用反转录聚合酶链反应(RT-PCR)检测了40例活动期SLE患者和20名正常人PMNS中5-LO的表达水平。结果:SLE患者5-LO的阳性表达率与正常对照组相比差异无统计学意义;但活动期SLE患者PMNS中的5-LOmRNA的平均表达水平明显高于非活动期SLE和正常对照组,而非活动期SLE患者与正常对照组间5-LO的平均表达强度无显著差异。结论:活动期SLE患者中5-LO参与了SLE的发病过程,为特异性5-LO抑制剂或CysLTs受体拮抗剂应用于SLE的治疗提供了理论依据。Objective： To investigate the role and significance of 5-lipoxygenase （5-LO） in the pathogenesis of systemic lupus erythematosus （SLE）. Methods： Applying reverse transcription-polymerase chain reaction （RT-PCR） Technique to semiquantitatively analyze 5-LO mRNA expression in the peripheral Polymorphomuclear leukocytes （PMNs） from 40 SLE patients and 20 healthy subjects. Results： Compared with normal controls, there are no statistical difference in the positive rates of 5-LO in SLE. The mean levels of 5-LO mRNA expression in active SLE group were significantly higher than those in non-active SLE group and normal controls respectively. However, there are no significant difference in the mean level of 5-LO mRNA expression between patients with non -active SLE group and normal controls. Conclusion： The levels of 5-LO mRNA expression in active SLE group increase karkedly, which may be involved in the pathogenesis of SLE and provide a valuable scientific basis for the clinical use of 5-LO inhibitors and cysteinyl leukotrienes receptor antagonist.

关 键 词：红斑狼疮 系统性 聚合酶链反应 5-脂氧化酶 

分 类 号：R593.24[医药卫生—内科学]