牛羊胆汁中甘氨胆酸提取纯化工艺研究  

STUDIES ON EXTRACTION AND PURIFICATION TECHNIQUES OF GLYCOCHOLIC ACID FROM BOVINE AND SHEEP BILE

作　　者：赵珍[1] 李培锋[1] 李慧峰[1] 关红[1] 李玉娟[2] 

机构地区：[1]内蒙古农业大学动物科学与医学学院,呼和浩特010018 [2]包头农牧学校,包头014300

出　　处：《内蒙古农业大学学报（自然科学版）》2006年第2期9-12,共4页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：内蒙古自治区人才基金项目

摘　　要：采用盐析、萃取、柱层析等生物化学方法,从牛、羊胆汁中提取出了有效成分甘氨胆酸,并采用薄层层析法及薄层扫描法进行定量、定性检测,测得甘氨胆酸的纯度可达98%以上,牛羊胆汁甘氨胆酸的回收率分别为64%和62%。Glycocholic Acid（GCA） was extracted and purified from Bovine and sheep bile by using the biochemistry techniques such as salting - out, extraction, dehydration, silica gel separation, and the qualitative and quantitative analysis of GCA were performed by using thin-layer chromatography. The purity of GCA was reached to 98% ,and reclaim rate was 64% and 62% .

关 键 词：牛胆汁 甘氨胆酸 提取 分离纯化 

分 类 号：S852.23[农业科学—基础兽医学]