转兔防御素基因小球藻培养的营养模式研究  

Effect of culture modes on the growth of transgenic Chlorella and rabbit defensin expression

作　　者：韩兴梅[1] 李元广[1] 魏晓东[1] 李兴武[1] 孙勇如[2] 王义琴[2] 

机构地区：[1]华东理工大学海洋生化工程研究所生物反应器工程国家重点实验室,上海200237 [2]中国科学院遗传与发育生物学研究所,北京100101

出　　处：《海洋科学》2006年第7期48-52,共5页Marine Sciences

基　　金：国家863计划海洋生物技术主题资助项目(2002AA629110)

摘　　要：在250mL摇瓶中研究了光自养、异养、混合营养3种营养模式对转兔防御素基因(NP-1)小球藻(Chlorella)的生长、NP-1表达量、总蛋白质含量、pH、葡萄糖消耗以及光衰减等几个方面的影响。结果表明,异养培养为转NP-1基因小球藻的最适营养模式。在此基础上,采用5,15,30L生物反应器补料分批异养培养转NP-1基因小球藻,细胞质量浓度分别达到3.67,4.87,6.39g/L。The influences of culture modes including autotrophy, heterotrophy, mixotrophy on process characteristics of transgenic Chlorella with rabbit defensin （NP-1） gene in the 250mL flasks were investigated including cell growth, NP-1 expression, protein contents, pH, glucose consumption and light attenuation. The results showed that heterotrophy was the optimal culture mode for the transgenic Chlorella with NP- 1 gene. Based on these results, transgenic Chlorella was cultured in bioreactors of 5, 15, 30L and the corresponding cell densities were 3.67, 4.87, 6.39g/L, respectively.

关 键 词：转基因小球藻 兔防御素 营养模式 表达 

分 类 号：Q949.2[生物学—植物学]