高效液相色谱法测定妇康宁片中芍药苷的含量  

Determination of Paeoniflorin in Fukangning Tablets by HPLC

作　　者：白岩[1] 李伯军[1] 李铁强[1] 吴凯莹[2] 杨荣艳[1] 

机构地区：[1]吉林省四平市药品检验所,136000 [2]吉林省四平市卫生学校

出　　处：《中国药事》2006年第7期416-417,427,共3页Chinese Pharmaceutical Affairs

摘　　要：采用高效液相色谱法测定妇康宁片中芍药苷的含量。色谱柱：Agilent Extend ODS C18；流动相：甲醇-水-冰醋酸（25：75：0.2）；流速：1．0ml·min^-1；检测波长：230nm。芍药苷在5-100μg·ml^-1浓度范围内，峰面积积分值与浓度呈良好的线性关系（r=0．9998）；平均回收率为99．71％，RSD=1．43％（n=6）。本方法简便、准确、重现性好。A HPLC method was established for determination of paeoniflorin in Fukangning tablets. An Agilent Extend ODS C18 column was used with methanol- water- glacial acetic acid （25 ： 75 ： 0. 2） as mobile phase. The flow rate was 1.0ml· min^-1 and detection wavelength was 230nm. There was a good linear relationship between peak area and concentration when paeoniflorin was 5-100μg ·ml^-1 （r= 0. 9998）. The average recovery was 99.71% and RSD=1. 43% （n=6）. The method was simple, accurate and reproductive.

关 键 词：妇康宁片 芍药苷 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]