一种含吲哚咪唑基Ru(Ⅱ)配合物的酸碱性质和与DNA相互作用的研究  

Study on the Acid-Base and DNA-Binding Properties of an Indole Group-Containing Ruthenium(Ⅱ) Complex

作　　者：赵琳[1] 吴宝燕[1] 高丽华[1,2] 王科志[1] 

机构地区：[1]北京师范大学化学系 [2]北京工商大学化学与环境工程学院北京100037

出　　处：《化学学报》2006年第13期1402-1406,共5页Acta Chimica Sinica

基　　金：国家自然科学基金(Nos.20371008;90401007);北京市自然科学基金(No.2052008);北京市优秀人才基金资助项目.

摘　　要：合成了一个新的Ru(II)配合物[Ru(bpy)2(H2iip)](ClO4)2?5H2O[bpy=2,2'-联吡啶,H2iip=2-吲哚基-咪唑并[4,5-?][1,10]-邻菲罗啉].通过酸碱滴定发射光谱测定了该配合物的表观电离常数;用紫外可见光谱、荧光光谱、稳态荧光淬灭、溴化乙锭竞争键合、粘度测量和DNA裂解实验研究了配合物与DNA的相互作用性质.结果表明配合物以经典的插入模式与DNA键合,键合常数Kb=(5.97±0.27)×105mol-1?L(50mmol/LNaCl).A novel ruthenium polypyridyl complex of [Ru（bpy）2（H2iip）]（ClO4）2·5H2O [bpy=2,2′-bipyridyl, H2iip = 2-indole-3-yl-imidazol[4,5-f] [ 1,10]phenanthroline], was synthesized and characterized by elemental analysis and ^1H NMR spectrum. The pH effects on the UV-visible and emission spectra of the complex were studied, and the three apparent ionization constants were derived. The binding of the complex to calf thymus （ct） DNA was investigated with electronic absorption and luminescence spectroscopic titrations, steady-state emission quenching, DNA competitive binding with ethidium bromide, viscosity measurements and thermal denaturation. The results indicated that the complex was bonded to ct-DNA through a classic intercalative mode with an intrinsic binding constant of （5.97±0.27）×10^5 mol^-1·L （50 mmol/L NaCl）.

关 键 词：钌(Ⅱ)配合物 DNA 插入作用 

分 类 号：O641.4[理学—物理化学] TQ460.35[理学—化学]