结合珠蛋白和铁蛋白在急性肺栓塞中的表达变化  

Expression changes in haptoglobin and ferritin after acute pulmonary embolism

作　　者：李圣青[1] 任新玲[1] 刘阿茹[1] 简文[1] 戚好文[1] 阙海萍[2] 张晓君[2] 赵馨[2] 潘刚[2] 杨树广[2] 刘少君[2] 

机构地区：[1]第四军医大学西京医院呼吸科,西安710032 [2]第四军医大学军事医学科学院三所一室,西安710032

出　　处：《解放军医学杂志》2006年第7期657-659,共3页Medical Journal of Chinese People's Liberation Army

摘　　要：目的研究急性肺栓塞时结合珠蛋白和铁蛋白的表达变化。方法采用RT-PCR方法检测急性肺栓塞大鼠模型肺组织中结合珠蛋白和铁蛋白mRNA水平的变化;采用Westernblot技术检测急性肺栓塞大鼠血清中结合珠蛋白和铁蛋白蛋白水平的变化;采用免疫散射比浊法测定58位肺栓塞/深静脉血栓(PE/DVT)患者和40例对照(对照组)血清中的结合珠蛋白和铁蛋白的蛋白水平。结果急性肺栓塞后大鼠肺组织中结合珠蛋白和铁蛋白mRNA水平逐渐升高,血清中结合珠蛋白和铁蛋白的蛋白水平也逐渐升高。PE/DVT病人血清中结合珠蛋白的蛋白浓度为2063·48±425·38mg/L,对照组为824·37±235·24mg/L(P<0·01);PE/DVT患者血清中铁蛋白的蛋白浓度为554·43±136·18μg/L,对照组为79·42±31·57μg/L(P<0·01)。结论急性肺栓塞后肺组织细胞增加了结合珠蛋白和铁蛋白的合成,导致这两种蛋白的血清水平明显升高,表明结合珠蛋白和铁蛋白对PE/DVT病人具有一定的诊断价值。Objective To study the expression changes in haptoglobin and ferritin after acute pulmonary embolism （PE）. Methods A rat model of acute PE was reproduced in the present study by injecting 3-4 thrombi into the left jugular vein. The total RNA of the lung tissue was obtained at different time points, i.e. lh, 8h, 24h, and 48h, and the normal rats were used as control. The changes in mRNA level of haptoglobin and ferritin were determined by semi-quantitative RT-PCR, and the changes in serum protein level of haptoglobin and ferritin were assessed by Western blot methods. Finally the serum concentrationa of haptoglobin and ferritin were determined by means of immunonephelometry in 58 PE/deep vein thrombosis （PE/DVT） patients and 40 healthy individuals （comrols）. Results The mRNA levels of haptoglobin and ferritin were increased gradually in the experimental animals compared with controls, and their serum protein levels were also elevated in the rat with acute PE. The concentration of serum ferritin in controls was 79. 42±31.57μg/L, whereas in PE/DVT patients it was 554. 43±136. 18μg/L （P〈0. 01）. The concentration of serum haptoglobin in controls was 824. 37±235. 24 mg/L, whereas in PE/DVT patients it was 2 063. 48±425.38mg/L （P〈0. 01）. Candusion The synthesis of haptoglobin and ferritin was increased in the lung cells after acute PE, resulting in up-regulation of their serum protein levels in patients with PE. The results of study of changes in their expression suggest that haptoglobin and ferritin are of great value in the diagnosis of PE/DVT.

关 键 词：肺栓塞 铁蛋白 触珠蛋白类 印迹法 蛋白质 逆转录聚合酶链反应 

分 类 号：R563.5[医药卫生—呼吸系统]