猪圆环病毒PCR检测方法的优化  

Optimization of PCR for the Detection of Porcine Circovirus2

作　　者：董志强[1] 张志[2] 李晓成[2] 陈德坤[1] 张燕霞[2] 黄保续[2] 

机构地区：[1]西北农林科技大学,陕西杨凌712100 [2]农业部动物检疫所,山东青岛266032

出　　处：《中国病毒学》2006年第4期405-407,共3页Virologica Sinica

摘　　要：Three pairs of primers P1/P2,P3/P4 and P5/P6 of porcine circovirus 2 were designed and synthesized to compare the sensitivity and specialization to amplify products of 404bp,647bp and 1773 bp.Optimum dilution of PK15 cell genome infected with PCV 2 was achieved with 10-6ng,10-5ng to yield from the three pairs of primers.It was also confirmed that P1/P2 were 68.4% successful in detecting PCV2 in 57 swine samples from the province of Fujian,The other primers,P3/P4,P5/P6,were only 57.9% and 8.7% successful,respectively.We conclude that p1/p2 is the optimum primer pair for surveying incidents of PCV2 in pigs.Three pairs of primers P1/P2, P3/P4 and P5/P6 of porcine circovirus 2 were designed and synthesized to compare the sensitivity and specialization to amplify products of 404bp, 647bp and 1773 bp. Optimum dilution of PK15 cell genome infected with PCV 2 was achieved with 10^-6ng, 10^-5ng to yield from the three pairs of primers. It was also confirmed that P1/P2 were 68.4% successful in detecting PCV2 in 57 swine samples from the province of Fujian, The other primers, P3/P4, P5/P6, were only 57.9% and 8.7% successful, respectively. We conclude that pl/p2 is the optimum primer pair for surveying incidents of PCV2 in pigs.

关 键 词：圆环病毒 PCR 检测方法 优化 

分 类 号：S854.43[农业科学—临床兽医学]