水稻矮缩病毒昆明分离物抗血清制备及免疫捕捉PCR检测  

Antiserum Preparation against Rice Dwarf Virus Kunming Isolate and Detection by Immunocapture PCR Tests

作　　者：彭潞波[1] 李婷婷[1] 魏春红[2] 方琦[1] 董家红[1] 张丽珍[1] 苏晓霞[1] 丁铭[1] 张仲凯[1] 李毅[2] 

机构地区：[1]云南省农业科学院生物技术及种质资源研究所,云南昆明650223 [2]北京大学生命科学学院,北京100871

出　　处：《中国病毒学》2006年第4期408-410,共3页Virologica Sinica

基　　金：国家重点基础研究发展规划项目(G2000016201)

摘　　要：By Using the modified method to extract Rice dwarf virus virions from infected rice leaves,a concentration 3.854mg/mL of purified virus virions reached,and the Rabbits was immunized with purified virions,yielding high titer antiserum;1:20480 by the indirect enzyme-linked immunosorbent assay(I-ELISA)test.The antiserum successfully detected three rice samples from Kunming,Yuxi and Luliang by I-ELISA.Immunocapture polymerase chain reaction(IC-PCR),respectively was the utilized to show that serologic and molecular methods were successfully used for detection of Rice dwarf virus.By Using the modified method to extract Rice dwarf virus virions from infected rice leaves, a concentration 3.854mg/mL of purified virus virions reached, and the Rabbits was immunized with purified virions, yielding high titer antiserum; 1：20480 by the indirect enzyme-linked immunosorbent assay （I-ELISA） test. The antiserum successfully detected three rice samples from Kunming, Yuxi and Luliang by I-ELISA.Immunocapture polymerase chain reaction （IC-PCR）, respectively was the utilized to show that serologic and molecular methods were successfully used for detection of Rice dwarf virus.

关 键 词：水稻矮缩病毒 抗血清 间接ELIZA 免疫捕捉PCR 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]