大豆耐盐相关基因STL的克隆与分析  

Cloning and Characterization of a Salt-tolerance Related Gene from Glycine max

作　　者：李富鹏[1] 张凌[1] 王国丰[1] 曹又方[1] 王绛[1] 唐克轩[1,2] 

机构地区：[1]上海交通大学农业与生物学院植物生物技术研究中心,上海市生物技术重点实验室 [2]复旦大学生命科学学院遗传工程国家重点实验室,上海200433

出　　处：《分子植物育种》2006年第4期464-468,共5页Molecular Plant Breeding

基　　金：上海市科技兴农重点项目（农科攻字（2001）第3-2和30470919）资助.

摘　　要：利用RACE技术从大豆中克隆出一条耐盐相关基因STL(GenBank登录号为DQ234265),该基因全长为1286bp,其中编码区为717bp,编码一个由238个氨基酸组成的蛋白质。STL与拟南芥的耐盐蛋白STO(salttoleranceprotein,GenBank登录号NP_849598)具有63.6%的同源性。RT-PCR分析发现,当用1mol/LNaCl处理大豆叶片不同时间时,大豆STL的表达量并未发生显著变化,与拟南芥STO的表达模式相似,推测STL在大豆中也具有类似的耐盐功能。A salt-tolerance related gene STL （GenBank accession No. DQ234265） was isolated from Glycin max by rapid amplification ofcDNA ends （RACE）. The full-length cDNA of STL was 1 286bp and contained a 717 bp open reading frame （ORF） encoding 238 amino acids. Sequence analysis indicated that the deduced STL shared 63.6% identity with Arabidopsis thaliana salt tolerance protein STO （GenBank accession No. NP_849598）. RT-PCR analysis revealed that after treated with lmol/L NaC1 for different duration, STL expression level in all the tested plant tissues did not change obviously, similar with that of STO in A. thaliana, suggesting similar salt-tolerance mechanism may exist between Arabidopsis and soybean.

关 键 词：大豆 RACE STL(STO—Like) 

分 类 号：S565.1[农业科学—作物学]