普那菊苣高效再生体系建立和遗传转化研究  

High Efficient System Establishment on Plant Regeneration and Study on Genetics Transformation in Puna Chicory (Cichorium Intybus L.)

作　　者：宋书锋[1] 曹凤[2] 杨培志[3] 陈明利[2] 李传山[2] 郭蔼光[1,2] 

机构地区：[1]西北农林科技大学生命科学学院,杨凌712100 [2]陕西省农业分子生物学重点实验室,杨凌712100 [3]西北农林科技大学动物科技学院,杨凌712100

出　　处：《分子植物育种》2006年第4期565-570,共6页Molecular Plant Breeding

基　　金：西北农林科技大学博士创新基金资助.

摘　　要：普那菊苣为菊科多年生草本植物,从国外引种后已在我国得到广泛种植。普那菊苣草质柔嫩,适口性优良,营养丰富,牛、羊、猪、鱼、兔极喜食,是一种非常优良的牧草,具有广阔的发展前景。本研究通过对不同激素浓度配比实验,建立普那菊苣的高频再生体系,结果表明MS+6-BA2.0mg/L+IBA0.1mg/L培养基可高效诱导愈伤组织和芽的分化,1/2MS培养基可快速诱导根的生成,形成再生植株。通过构建植物表达载体pBI121-GFP,利用农杆菌介导转化普那菊苣以探寻高效的普那菊苣转化体系,结果显示以农杆菌LBA4404为介导菌株、以叶片为转化受体、3d预培养时间和3d共培养时间以及350mg/L羧苄青霉素和40mg/L卡那霉素筛选浓度转化效果较好,为利用普那菊苣生产动物口服型疫苗建立了初步的技术基础。Puna Chicory （Cichorium Intybus L.）, a perennial herbaceous plant of Compositae family, is widely planted at our country after intruduction. Puna Chicory is tender and dainty. It is rich of nourishment. Cattles, sheep, pigs, fish and rabbits prefer it. Puna Chicory is a kind of pasture that has a promotional value. This research discussed how to establish high-efficient regeneration system of Puna Chicory by the different phytohormone combination experiment. The result showed that the medium of MS＋6-BA 2.0mg/L＋IBA 0. 1mg/L could efficiently induce the differentiation of callus and bud of leaf, the medium of 1/2MS induced the regeneration of roots quickly, and the regenerated plant formed. Vector pBI121-GFP was constructed and transformed to Puna Chicory by Agrobacterium tumefaciens. The regenerated resistant plants could be obtained by pre-culmring for 3d, co-culturing with Agrobacterium tumefaciens LBA4404 for 3d, then transferring to the callus inducement media containing 350mg/L Carb and 40 mg/L Kan for 20d. This system is the elementary technology for production of mammal edible vaccine.

关 键 词：普那菊苣(Cichorium Intybus L.) 组织培养 根癌农杆菌 

分 类 号：S548[农业科学—作物学]