氟桂利嗪对小鼠生精细胞T型Ca^(2+)通道的作用  

Effects of Flunarizine on T-type Calcium Channels in Mouse Spermatogenic Cells

作　　者：王昌松[1] 高晓华[1] 程宏[1] 龚伟[2] 张习春[3] 程洁[1] 高蓉[1] 肖杭[1] 

机构地区：[1]南京医科大学应用毒理省重点实验室,江苏南京210029 [2]江苏省疾病控制中心,江苏南京210009 [3]复旦大学神经生物学系,上海200433

出　　处：《中华男科学杂志》2006年第7期594-597,601,共5页National Journal of Andrology

基　　金：国家自然科学基金项目(30170811;30571555);国家973基础研究重大资助项目(2002CB512908)

摘　　要：目的:研究二苯烷基氨类衍生物氟桂利嗪(F lu)对小鼠生精细胞T型钙电流(ICaT)的影响。方法:采用急性机械分离的小鼠生精细胞,应用膜片钳全细胞记录技术,观察F lu对ICaT的影响。结果:钳制电位为-90 mV、刺激电压为-30 mV时,F lu(0.1、1、10、100μmol/L)可抑制小鼠生精细胞Ca2+电流,抑制率分别为(23.34±2.76)%、(46.04±3.52)%、(62.52±3.70)%、(73.52±3.12)%(P<0.05);F lu对T型Ca2+通道的半数最大抑制浓度为0.29μmol/L。结论:F lu对生精细胞T型Ca2+通道有阻断作用,呈浓度依赖性;Cav3.2是生精细胞T型Ca2+电流的主要贡献者。Objective： To investigate the effects of Flunarizine （Flu） on T-type calcium currents （ ICaT ） in spermatogenic ceils. Methods： Ca^2＋ currents were obtained in acutely dissociated mouse spermatogenic cells using whole-cell patch clamp technique and the effects of Flu on ICaT were observed. Results： Flu of 0.1,1,10,100 μmoL/L inhibited ICaT in mouse spermatogenic cells significant- ly with the K50 value of 0.289 μmoL/L（ P 〈 0.05 ）. With the holding potential at -90 mV and stimulating potential at -30 mV, the inhibition rates of Flu were （23.34±2.76）%, （46.04±3.52）%, （62.52±3.70）% and （73.52 ±3.12）%, respectively. Conclusion ： Flu has significant inhibitory effects on ICaT in mouse spermatogenic cells, with concentration dependence. Ca,3.2 is the main contributor to T-type Ca^2＋ currents in spermatogenic cells.

关 键 词：氟桂利嗪 T型Ca^2+通道 生精细胞 小鼠 

分 类 号：R96[医药卫生—药理学]