灯盏花素对脑死亡巴马小型猪肺脏形态变化与PKC-α mRNA及其蛋白表达的影响  被引量:1

Effects of breviscapine on the morphologic change of lung and PKC-α mRNA and its protein expression in brain-dead BA-Ma mini pigs

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作  者:吴阳[1] 张弓[1] 李震[1] 唐哲[1] 赵永福[1] 张水军[1] 

机构地区:[1]郑州大学第一附属医院普外科,河南郑州450052

出  处:《复旦学报(医学版)》2006年第4期530-534,共5页Fudan University Journal of Medical Sciences

基  金:河南省杰出人才创新基金(0421002500)资助

摘  要:目的探讨灯盏花素对脑死亡状态下巴马小型猪肺脏PKC信号途径及肺脏形态变化的影响。方法健康巴马小型猪15只,随机分为3组:脑死亡组(B组)、灯盏花素处理组(D组)及对照组(C组),每组5只。采用改进的缓慢间断颅内加压法建立脑死亡模型。分别于脑死亡后第3、6、12、18和24h检测血清中的TNT-α、IL-1β和IL-6水平。于脑死亡后第24h取肺脏组织,HE染色观察肺脏组织变化,免疫组化染色观察PKC-α蛋白的表达水平,RT-PCR法检测PKC-αmRNA的表达水平,电镜观察肺脏超微结构变化。结果(1)与对照组相比,脑死亡组及灯盏花素处理组血清中IL-1β、IL-6、TNF-α水平自脑死亡后3h开始升高,并随时间的延长而逐渐升高,但灯盏花素处理组上述指标升高幅度显著低于脑死亡组(P<0.05);(2)与对照组相比,脑死亡组及灯盏花素组肺组织中PKC-αmRNA及其蛋白水平在24h均升高,且常规病理及超微结构可见形态学发生改变,但灯盏花素处理组上述指标升高幅度显著低于脑死亡组(P<0.05)。结论灯盏花素通过抑制肺脏PKC-α信号转导途径而减少炎症介质的释放,可能是其保护脑死亡状态下肺脏的形态及结构的重要机制之一。Purpose To investigate the effects of breviscapine on the morphologic change of lung and PKC-α mRNA and its protein expression in brain-dead BA-Ma mini pigs. Methods Fifteen BA-Ma mini pigs were randomized into 3 groups; brain-dead group (group B), breviscapine pretreatment group (group D), and control group (group C), 5 pigs in each group. The brain-dead models were established by increasing intracranial pressure in a modified, slow and intermittent way. At 3, 6, 12, 18 and 24 h after the initial brain death, serum TNF-α, IL-1β, and IL-6 were determined. At 24 h, tissues of lung were taken, the changes of tissues of lung were observed by HE staining, the expression of PKC-α by immnohistochemistry, and PKC-α mRNA by RT-PCR. The ultrastructure changes of lung cells were observed under electron microscope. Results (1) Compared with group C, serum TNF-α, IL-1β, and IL-6 in group B and D began to increase since 3h after the initial brain death and ascended gradually along with time, but the parameters for group D were less severe than those in group B (P〈0. 05) ; (2) Compared with group C, changes of PKC-α mRNA and expression of PKC-α protein in lung cells in group B and D increased at 24 h and morphological changes of lung cells could be found, but the parameters for group D were less severe than those in group B (P〈0.05). Conclusions Breviscapine can inhibit the degree of PKC-α mRNA transcription and protein translation, decrease the release of inflammatory factors, and these function are supposed to be one of the mechanisms of protecting the morphologic and structure of lung during brain death.

关 键 词:灯盏花素 巴马小型猪 脑死亡 蛋白激酶C 

分 类 号:R392.32[医药卫生—免疫学]

 

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