T细胞激活剂对Jurkat细胞重组激活基因表达的影响  

Effects of T cell activators on mRNA expression of recombination-activating genes in Jurkat cells

作　　者：邹红云[1] 马骊[1] 姚新生[1] 温茜[1] 王小宁[2] 

机构地区：[1]南方医科大学生物技术学院分子免疫学研究所,广东广州510515 [2]华南理工大学生物科学与工程学院,广东广州510641

出　　处：《第四军医大学学报》2006年第14期1253-1255,共3页Journal of the Fourth Military Medical University

基　　金：国家重点基础研究发展规划"973"项目资助(2001CB510008)

摘　　要：目的:研究T细胞激活剂PHA,抗CD3mAb,PMA+A23187对人T细胞白血病细胞株Jurkat重组激活基因(RAGs)mRNA表达水平的影响.方法:采用RTPCR法检测不同T细胞激活剂作用不同时间后Jurkat细胞中RAG1,RAG2mRNA,以β肌动蛋白(βactin)的表达作为内参照进行灰度分析,比较不同组Jurkat细胞RAGsmRNA的表达水平.结果:三种T细胞激活剂刺激诱导的Jurkat细胞RAG1mRNA表达量明显低于对照组(P<0.05),且RAG1mRNA表达量的下降与刺激诱导作用时间有关;PHA刺激诱导后RAG2表达量显著低于对照组(P<0.05);PMA+A23187刺激诱导6,12h均未检测出RAG2mRNA的表达;而抗CD3mAb作用12h组RAG2mRNA表达量显著高于对照组(P<0.05).结论:Jurkat细胞同时表达RAG1,RAG2mRNA,并在一定诱导下RAGs表达量发生改变,因此有可能成为研究外周T细胞RAGs表达调节的一个潜在的细胞模型.AIM： To investigate the effects of T cell activators on the mRNA expression of recombination-activating genes （RAGs） in Jurkat cells. METHODS： After Jurkat cells were treated with PHA （ 20 mg/L）, anti-CD3 mAb （ 1 ： 100 ）, PMA （40μg/L） ＋ A23187 （0.5 μmol/L） for6 and 12 h respectively, RAG-1 and RAG-2 mRNA were measured by RT-PCR. Semiquantitative analysis was used to evaluate RAG-1 and RAG-2 mRNA levels in different groups. REULTS： Compared with the control groups, the levels of RAG-1 mRNA were significantly lower in different treated groups （ P 〈 0.05 ）, and RAG-1 mRNA levels were associated with treatment time. RAG-2 mRNA levels in PHA treated groups were significantly lower than those in the control groups （ P 〈 0.05）. No RAG-2 mRNA was detected in PMA ＋ A23187 treated groups. However, RAG-2 mRNA level was significantly higher in anti-CD3 mAb （ 12 h） treated group than that in control groups（ P 〈 0.05 ）. CONCLUSION： RAG-1 and RAG-2 mRNA were coexpressed in Jurkat cells and could be regulated by T cell activators. The results may give a clue that Jurkat cells maybe provide an ideal cell line model for studying the regulation of RAGs in peripheral T cells.

关 键 词：T细胞激活剂 JURKAT细胞 重组激活基因 逆转录聚合酶链反应 

分 类 号：R338[医药卫生—人体生理学]