激素联合内毒素所致骨坏死微循环障碍超微结构观察  

Ultrastructural examination for microcirculatory disturbance in glucocorticoid and LPS induced osteonecrosis

作　　者：张德志[1] 胡蕴玉[1] 孟国林[1] 袁志[1] 毕龙[1] 刘丹平[2] 

机构地区：[1]第四军医大学西京医院骨科研究所,陕西西安710033 [2]锦州医学院附属第一医院骨科,辽宁锦州121001

出　　处：《第四军医大学学报》2006年第14期1279-1281,共3页Journal of the Fourth Military Medical University

摘　　要：目的:观察激素联合内毒素所致骨坏死骨内微血管内皮表面的超微结构损害和血栓形成.方法:实验动物分为模型组和正常对照组.模型组前2d间隔24h于静脉缓慢注射脂多糖(LPS),每次剂量30μg/kg;第3～8日静脉注射地塞米松,1次/d,剂量7.5mg/kg.对照组给予生理盐水0.5mL/kg.满8wk时取材前,股骨头压力灌注肝素钠、100mL/L中性甲醛固定液.一半股骨头标本制备脱钙切片,苏木素伊红染色,观察骨坏死情况.另一半标本制备不脱钙切片,脱掉包埋剂,临界点干燥、喷金、扫描电镜观察软骨下微血管内皮表面超微结构改变.结果:模型组股骨头软骨下微血管内皮表面凹凸不平,管腔不规则,可见管腔填塞、血栓形成;此结果与组织学观察结果一致.结论:激素联合内毒素所致骨坏死微循环障碍的主要表现为微血管内皮损伤和微血栓形成.AIM： To observe the ultrastructure of intraosseous vascular endothelial damage and thrombosis in a glucocorticoid and LPS induced osteonecrosis in rabbits. METHODS： Sixteen New Zealand white rabbits were divided into osteonecrosis model group （n=10） and control group （n=6）. In model group, LPS （30 μg/kg） was given intravenously twice at an interval of 24 h, and then, 24 h after the second injection of LPS, dexamethasone （7.5 mg/kg） was given 5 times to the animals at an interval of 24 h. Saline infusion （0.5 ml/kg） was given in the control group. After pressurized perfusion with heparin-saline and 10% formalin to hind limbs, the femoral head specimens were retrieved at week 8 postoperatively. Decalcified sections were prepared from half of the femoral heads, stained with hematoxylin and eosin, examined histologically for osteonecrosis. Undecalcified sections were prepared from the other half of the specimens, and then they were cleared of embedding material and were dried at critical-point and coated with gold-palladium in a high-vacuum evaporator. The sections were examined under scanning electron microscope for observing uhrastructural changes of the surface of microvascular endothelium in subchondral bone. RESULTS： The scanning electron microscope revealed irregular lumen, rough surface of microvascular endothelium or tamponade, or thrombosis of the subchondral vessels in model group, which were consistent with the features of histological examination. CONCLUSION： Endothelium damage and thrombosis of the subchondral vessels were the important features in the development of microcirculatory disturbance in glucocorticoid and LPS induced osteonecrosis.

关 键 词：超微结构观察 骨坏死 脂多糖类 糖皮质激素类 微循环障碍 显微镜检查 电子 

分 类 号：R681[医药卫生—骨科学]