注射型磷酸钙骨水泥/纤维蛋白胶作为BMP注射性载体的可行性研究  

Feasibility study of injectable calcium phosphate cement and fibrin sealant as an injectable carrier of bone morphogenic protein

作　　者：刘通方[1] 刘建[1] 孟国林[1] 袁志[1] 吕荣[1] 毕龙[1] 王登虎[1] 刘艳武[1] 胡蕴玉[1] 

机构地区：[1]第四军医大学西京医院全军骨科研究所,陕西西安710033

出　　处：《第四军医大学学报》2006年第14期1296-1299,共4页Journal of the Fourth Military Medical University

摘　　要：目的:了解以注射型磷酸钙骨水泥(ICPC)/纤维蛋白胶(FS)共同作为骨形态发生蛋白(BMP)复合载体的注射型骨修复材料在小鼠体内异位成骨的能力,探讨其作为注射性骨替代物的可行性.方法:在小鼠肌袋随机注射或植入ICPC/FS/bBMP复合物、ICPC/FS复合物和单纯bBMP材料,术后2wk,4wk取材,通过碱性磷酸酶测定和组织学检查,观察其异位诱导成骨能力.并对术后4wk时新生骨进行定量测定和统计学比较.结果:ALP和组织学检查结果表明ICPCFS/bBMP复合物有较多新骨形成,单纯bBMP诱导出少量骨ICPC/FS复合物未见新生骨,诱导骨量有统计学意义(P<0.05).结论:可注射性ICPC/FS/BMP复合物具有良好的骨诱导活性,ICPC/FS是BMP的良好注射性载体之一.AIM： To evaluate the feasibility of injectable calcium phosphate cement （ICPC） and fibrin sealant （FS） as an injectable compound carrier of bone morphogenic protein （BMP） .METHODS： The composites of ICPC/FS/bBIdP, ICPC/FS or bBMP were injected subcutanously or implanted into thigh muscle pouches of 45 mice at random. The effect of ectopic bone formation was evaluated by biochemical examination of alkaline phosphatase （ALP） and histological examination 2 and 4 weeks after operation and the new bones were weighed in the 4th postoperative week to compare statistically the ability of bone formation of different composites. RESULTS： ICPC/FS/bBMP composite induced the formation of new bone most effectively. BBMP stimulated tess asteogenesis while ICPC/FS showed no sign of new bone formation. There was a significant difference among them （ P 〈 0.05 ）. CONCLUSION： The injectable bone substitute, ICPC/FS/ BMP, exhibited an excellent osteoinductive activity, and ICPC/FS was a good carrier for BMP.

关 键 词：注射剂 磷酸钙类 骨粘合剂 纤维蛋白组织粘着剂 骨形态发生蛋白质类 载体 骨诱导 

分 类 号：R318.08[医药卫生—生物医学工程]