CD14基因启动子多态性与冠心病的关系  

Association between CD14 gene promo-ter region polymorphisms and coronary heart disease

作　　者：韦叶生[1] 刘运广[1] 唐任光[1] 蓝景生[2] 

机构地区：[1]右江民族医学院附属医院临床检验中心 [2]右江民族医学院附属医院心血管内科,广西百色533000

出　　处：《第四军医大学学报》2006年第7期630-633,共4页Journal of the Fourth Military Medical University

基　　金：广西壮族自治区青年科学基金(0447060)

摘　　要：目的:研究白细胞分化抗原14(CD14)基因启动子159C/T,260C/T多态性各等位基因及基因型在冠心病患者中的分布频率,分析CD14基因型及血清CD14水平与冠心病的相关性.方法:采用聚合酶链反应限制性片段长度多态性(PCRRFLP)技术,检测246例冠心病患者及258名正常人对照组CD14的基因多态性,同时采用酶联免疫吸附试验(ELISA)检测血清CD14水平.结果:冠心病组血清CD14水平显著高于对照组(P<0.01),CD14基因159C/T多态性在冠心病组和正常人群中的分布差异无显著性(P>0.05),而CD14基因260C/T多态性在两组人群中的分布差异存在显著性(P<0.05),等位基因频率的相对风险分析发现,T等位基因携带者患冠心病的风险是C等位基因的1.417倍(OR=1.417,95%CI:1.106～1.816),携带T等位基因的冠心病患者血清CD14水平显著高于不携带者(P<0.05).结论:CD14基因启动子260C/T多态性与冠心病的发病具有相关性,其中T等位基因可能是冠心病发病的遗传易感基因;携带T等位基因的个体可能通过促进CD14的高度表达进而增加了冠心病的发病风险.AIM： To study the allele frequencies and genotype distribution of CD14 gene promoter region -159C/T, -260C/T polymorphisms in Chinese patients with coronary heart disease （CHD）, and to analyze the association between the serum levels and genotypes of CD14 and CHD. METHODS： The polymorphisms of CD14 gene were analyzed by the polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） methods in 246 patients with CHD and 258 healthy controls, and the serum levels of CD14 were determined by enzyme-linked immunosorbent assay （ELISA）. RESULTS： The CHD group showed significantly higher serum levels of CD14 than control group （ P 〈 0.01 ） ; The distribution of CD14 gene - 159C/T polymorphism was not different between CHD group and control group （ P 〉 0.05 ） ; but the CD14 gene - 260C/T polymorphism was significantly different （P 〈 0.05 ）. The relative risk for CHD in T allele carriers was 1. 417 times as high as that in C allele carriers （OR = 1.417,95% CI：1. 106 - 1. 816）, and the serum level of CD14 in T allele carriers was significantly higher than that in non-carriers （P 〈 0. 05 ）. CONCLUSION： CD14 gene promoter region -260C/T polymorphism is associated with CHD; and T allele may be a risk factor for CHD; so T allele carriers have a higher risk for CHD by increasing the CD14 expression.

关 键 词：冠状动脉疾病 CD04 基因多态性 

分 类 号：R792.13[医药卫生—临床医学]