内皮细胞损伤、血小板活化及功能状态与2型糖尿病的相关性  

Correlation between vascular endothelial cell injury, platelet activation and functional status in type 2 diabetes

作　　者：王芳[1] 刘征雁[2] 牛保华[3] 

机构地区：[1]河南大学医学院检验系,河南开封475001 [2]河南大学医学院中医教研室,河南开封475001 [3]河南大学医学院病理教研室,河南开封475001

出　　处：《第四军医大学学报》2006年第7期637-638,共2页Journal of the Fourth Military Medical University

基　　金：河南省科技攻关基金(0424410102)

摘　　要：目的:观察2型糖尿病患者内皮细胞损伤、血小板活化及功能状态的变化及临床意义.方法:利用双抗体夹心ELISA法测定血浆血管性血友病因子抗原vWF∶Ag,血小板膜表面α颗粒膜蛋白GMP140水平;采用玻珠柱法测定血小板黏附率PAdT.结果:糖尿病组血浆vWF∶Ag(145.6±25.6)%,GMP140(16.8±3.5)μg/L,PAdT(80.3±5.3)%水平显著高于对照组(P<0.05);有血管病变的患者血浆vWF∶Ag(160.8±35.6)%,GMP140(18.9±5.5)μg/L,PAdT(89.5±7.8)%水平显著高于无血管病变组(P<0.05);相关分析显示,GMP140,PAdT水平与血浆vWF∶Ag水平显著正相关(r=0.446,0.413,P均<0.05).结论:2型糖尿病患者均存在血管内皮损伤,并由此引起血小板活化及血小板功能状态的改变;血浆vWF:Ag,GMP140及PAdT水平在一定程度上可反映2型糖尿病患者血管病变的情况.AIM： To investigate the vascular endothelial cell injury and the platelet activation and functional status change in type 2 diabetes patients. METHODS： The plasma levels of von willebrand factor： antigen （ vWF： Ag） and granule membrane protein-140（GMP-140） were measured by double sandwich ELISA. The adhesiveness of platelet was determined by Salzman＇s method. RESULTS： The plasma levels of vWF：Ag（ 145.6 ± 25.6）% , GMP-140（16.8 ±3.5）μg/L and PAdT （80.3 ±5.3）% of type 2 diabetes were significantly higher than those of normal controls （P 〈 0.05 ） ; The plasma levels of these markers in the group of type 2 diabetes with vascular complication were higher than those in the one without vascular complication （ P 〈 0. 05 ） ; plasma levels of GMP-140 and PAdT were positively correlated with those of vWF：Ag[ （160.8 ±35.6）%,（18.9 ±5.5） μg/L, （89.5 ± 7.8）%, r =0.446, 0.413, P〈0.05]. CONCLUSION： Type 2 diabetes can cause vascular endothelial cell injury, platelet activation and functional status change. The plasma levels of vWF：Ag, GMP-140 and PAdT may reflect vascular pathological changes in type 2 diabetes patients to a certain extent.

关 键 词：糖尿病 内皮损伤 血小板活化 血管病变 

分 类 号：R446.1[医药卫生—诊断学]