心肌肽素对豚鼠心室肌细胞L型钙通道的影响  

Effects of cardiomyopeptidin on L-type calcium channels in ventricular myocytes of guinea pigs

作　　者：吴辉[1] 李立环[1] 陈雷[1] 黄爱杰[1] 

机构地区：[1]中国医学科学院.中国协和医科大学阜外心血管病医院麻醉科,北京100037

出　　处：《中国药理学通报》2006年第5期611-615,共5页Chinese Pharmacological Bulletin

摘　　要：目的研究心肌肽素对豚鼠心室肌细胞L型钙通道的影响,探讨心肌肽素在离子通道水平的药理作用机制。方法用急性酶解分离法获得豚鼠心室肌细胞,标准的全细胞膜片钳技术记录L型钙电流(ICa-L)。结果心肌肽素1、5、10、50、100、500 mg.L-1使豚鼠心室肌细胞ICa-L分别增加(5±4)%、(21±5)%、(30±5)%、(55±8)%、(76±11)%、(80±9)%,半最大效应浓度(EC50)为(18±6)mg.L-1。心肌肽素50 mg.L-1使ICa-L激活时间(TTP)从(6.7±0.9)m s缩短为(5.9±0.7)m s(P<0.01);使ICa-L电流密度-电压曲线下移,但激活电压、峰电压和I-V曲线的形状不变;激活曲线向负电压方向变化,半数激活电压从(-4.3±0.4)mV减少至(-8.6±0.4)mV(P<0.05);不影响稳态失活曲线和稳态失活后恢复曲线。结论心肌肽素浓度依赖性增强豚鼠心室肌细胞ICa-L。Aim To determine the effect of cardiomyopeptidin on L-type calcium channels in ventricular myocytes of guinea pigs and to explore the mechanism of cardiomyopeptidin on the ionic channel level. Methods Single ventricular myocytes of guinea pigs were obtained with enzymatic dissociation method. The whole-cell patch-clamp recording technique was used to record the change of L-type calcium currents （ICa-L）. Results Cardiomyopeptidin （ 1,5, 10, 50, 100 and 500 mg · L-1） increased /Ca.L in a dose-dependent manner, the increasing rates were （ 5 ± 4 ） %, （21 ±5）%, （30 ±5）%, （55 ±8）%, （76±11）% and （80 ± 9 ） % respectively. The half maximal effect concentration （EC50） was （18 ±6） mg · L-1. In the presence of cardiomyopeptidin （50 mg · L-1）, the time to peak （TTP） of ICa-L was decreased from （6. 7 ±0. 9） ms to （5.9 ±0. 7） ms density-voltage curve of ICa-L （ P 〈 0. 01 ）. The current was moved down in the presence of cardiomyopeptidin 50 mg · L-1, but the active potential, the peak potential, and the shape of the curve had no changes. The activation curve of ICa-L was moved to more negativepotential in the presence ofcardiomyopeptidin （50 mg · L-1 ）, with a half active potential （ V0. 5 ） of （ - 4. 3 ± 0. 4 ） mV compared with that of （ - 8.6 ± 0. 4） mV in the control （P 〈 0. 05 ）. The steady inactivation curve of ICa-L and the steady inactivation recovery curve of ICa-L were not affected by cardiomyopeptidin（50 mg · L-1 ）. Conclusion Cardiomyopeptidin increases ICa-L of guinea pig ventricular myocytes in a concentration-dependent manner.

关 键 词：心肌细胞 L型钙通道 肽类 心肌肽素 

分 类 号：R-332[医药卫生] R322.11