IL-12体外促进慢性乙型肝炎患者PBMC IFN-γ的产生  

IL-12 promotes the cellular immunity of PBMC from patients with chronic hepatitis B virus infection in vitro

作　　者：熊仕秋[1] 刘惠平[2] 高翔[3] 林炳亮[4] 吴长有[1] 

机构地区：[1]中山大学基础医学院免疫学教研室,广州510089 [2]中国人民解放军458医院全军肝病中心,广州510600 [3]中山大学第一附属医院消化内科,广州510089 [4]中山大学第三附属医院传染科,广州510630

出　　处：《中国免疫学杂志》2006年第5期473-476,479,共5页Chinese Journal of Immunology

基　　金：国家重点基础研究发展规划973项目(2001CB510007);中山大学"百人计划"启动基金资助

摘　　要：目的:探讨体外IL-12对慢性乙型病毒性肝炎患者细胞免疫功能的影响,为临床治疗慢性乙型病毒性肝炎提供理论依据。方法:慢性乙型肝炎患者PBMCs与HBsAg、HBsAg+IL-12、HBcAg或HBcAg+IL-12孵育后,利用酶联免疫吸附实验(ELISA)检测细胞培养上清中IFN-γ的含量;用酶联免疫斑点实验(ELISPOT)在单个细胞水平上检测PBMCs中IFN-γ产生细胞的频率;用流式细胞记数仪(FACS)检测PBMCs中IFN-γ+的细胞亚群。结果:当乙肝患者PBMCs与HBsAg或HBcAg孵育后,只有少数病例发生反应,产生低水平的IFN-γ。当IL-12加入细胞培养后,能显著地增加HBsAg或HBcAg诱导IFN-γ的产生;分泌IFN-γ的细胞频率显著增加;IFN-γ+的细胞主要包括CD8+T细胞和非T细胞。结论:IL-12体外能增强慢性乙型肝炎患者的细胞免疫应答。Objective：To determine the effect of IL-12 on the cellular immune response of PBMC from patients with chronic hepatitis B virus infection, and provide basic scientific information for clinic therapy of this disease. Methods： PBMCS were prepared from peripheral blood of individuals with chronic HBV infection and cultured in the presence or absence of HBsAg and HBcAg with or without IL-12. The level of IFN-γin culture supematants, the frequency of IFN-γ-preducing cells, and the subpopulation of IFN-γ-producing cells were detected by either ELLSA ,ELISPOT or FACS. Results：Leas than 30% patients and very low level of IFN-γ were observed when PBMCs were stimulated with HBsAg or HBcAg alone. Addition of IL-12 to the cultures resulted in significant increase in IFN-γproduction and IFN-γ-producing cells. In addition, IL-12 induced expression of IFN-γ not only by CD8^＋T cells, but also by non-T cell populations. Conclusion：IL-12 can promote the cellular immune response to the chronic hepatitis B virus by the enhancement of IFN-γproduction.

关 键 词：白细胞介素12 慢性乙型病毒性肝炎 外周血单个核细胞 细胞免疫 

分 类 号：R392[医药卫生—免疫学]