先兆子痫患者外周血、脐血及蜕膜NK细胞的相关研究  

Study on NK cells in peripheral blood,umbilical blood and decidua of preeclampsia patients

作　　者：张展[1] 贾莉婷[1] 杨如镜[1] 侯磊[1] 常彩红[1] 郑芳[2] 张曦[1] 龚非力[2] 

机构地区：[1]郑州大学第三附属医院,郑州450052 [2]华中科技大学同济医学院免疫学系,武汉430030

出　　处：《中国免疫学杂志》2006年第5期477-479,共3页Chinese Journal of Immunology

基　　金：河南省教育厅科技攻关基金项目(524410061)

摘　　要：目的:对先兆子痫孕妇外周血、脐血及蜕膜NK细胞进行研究,以探讨先兆子痫的病因。方法:对42例先兆子痫患者及20例正常孕妇外周血、脐血NK细胞分离后记数。采用免疫组织化学法检测胎盘蜕膜NK表型后记数并检测其平均灰度。结果:①轻度及重度先兆子痫患者母血及脐血NK细胞数均显著高于正常孕妇(P<0·05);②重度先兆子痫患者蜕膜NK细胞数高于正常孕妇(P<0·05);③轻度及重度先兆子痫患者胎盘蜕膜NK细胞CD56组化染色平均灰度值高于正常孕妇(P<0·05)。结论:先兆子痫患者外周血、脐血及蜕膜的NK细胞数量均高于正常孕妇,且呈激活状态的胎盘蜕膜NK细胞较多,可能与发病有关。Objective：To analyze the change of the NK cells in peripheral blood, umbilical blood and deciduas of preeclampsia patients. Methods：The counts of the NK cells in peripheral blcod,umhilical blood and deciduas of preeclampsia patients（n =42） and normal late pregnant women（ n = 20） were detected. The immune indexes of the NK cells in decidua were detected by immunohistochemical. Results：The counts of the NK cells in peripheral blood and umbilical blood in precclampsia patients were significantly higher （ all P 〈 0. 05 ） than that in normal late pregnant women,while only in the deciduas of severe preeclampsia patients significantly higher （ P 〈 0. 05 ）. The value of CD56 NK cells in the decidua of preeclampsia patients was significantly higher（ P 〈 0. 05 ）. Conclusion： Both quantity and function of the NK cells in precclampsia patients became much more and stronger, respectively. It suggests that the NK cells may have relationship with the etiology of preeclampsia.

关 键 词：先兆子痫 NK细胞 蜕膜 人类白细胞抗原-G 

分 类 号：R714.24[医药卫生—妇产科学]