慢性阻塞性肺疾病大鼠肺内白细胞介素9和γ干扰素的表达  

The expressions of interleukin-9 and interferon-γ in the rat model of chronic obstructive pulmonary disease

作　　者：杨雪梅[1] 万毅新[1] 

机构地区：[1]兰州大学第二附属医院呼吸内科,甘肃兰州730030

出　　处：《中国呼吸与危重监护杂志》2006年第4期297-300,245,共5页Chinese Journal of Respiratory and Critical Care Medicine

摘　　要：目的观察大鼠烟雾暴露慢性阻塞性肺疾病(COPD)模型白细胞介素9(IL-9)和γ干扰素(IFN-γ)的表达,探讨IL-9和IFN-γ与COPD气道炎症的关系。方法单纯熏香烟法建立大鼠COPD模型。收集支气管肺泡灌洗液(BALF)行白细胞总数和分类计数检测;酶联免疫吸附法(ELISA)测定血清、BALF和肺组织中IL-9及IFN-γ的浓度;肺组织切片行HE染色观察形态学改变;采用图像分析系统定量测定肺平均内衬间隔(Lm)、平均肺泡数(MAN)和支气管壁厚/外径比值。结果(1)COPD组Lm及支气管壁厚/外径高于正常对照组,MAN低于正常对照组,差异有统计学意义(P均<0.01)。(2)COPD组血清、BALF和肺组织中IL-9浓度较正常对照组显著增高[血清:(4.02±2.12)pg/mL比(1.12±0.73)pg/mL;BALF:(5.73±2.60)pg/mL比(0.97±0.45)pg/mL;肺组织:(24.92±6.65)pg/mL比(11.42±4.23)pg/mL;P均<0.01]。COPD组血清、肺组织和BALF中IFN-γ的浓度与正常对照组比较均降低,前两者差异有统计学意义[血清:(21.54±7.65)pg/mL比(69.59±13.68)pg/mL;肺组织:(21.88±6.11)pg/mL比(69.84±15.35)pg/mL;P均<0.05]。(3)与正常对照组比较,COPD组BALF中白细胞总数[(5.29±1.61)×108/L比(2.10±1.19)×108/L,P<0.01]、巨噬细胞绝对计数[(4.42±1.38)×108/L比(1.84±0.93)×108/L,P<0.01]、中性粒细胞绝对计数[(1.32±0.36)×108/L比(0.093±0.043)×108/L,P均<0.01]均显著增高。(4)COPD组BALF中白细胞总数、巨噬细胞绝对计数、中性粒细胞绝对计数与BALF中IL-9浓度均呈正相关(r分别为0.9290、.835和0.924,P均<0.05),与肺组织中IL-9浓度呈正相关(r分别为0.8850、.983和0.775,P均<0.05),与血清中IL-9浓度无明显相关性。血清及BALF中IL-9浓度与IFN-γ无明显相关性,肺组织中IL-9与肺组织中IFN-γ则呈负相关(r=-0.818,P<0.05)。结论吸烟可使IL-9升高,IFN-γ水平降低,可能是吸烟导致COPD的原因之一。气道局部的IL-9表达与COPD气道炎症密切相关,可作为COPD气道炎症的检测指标之一。Objective To investigate the changes of interleukin-9 （IL-9） and interferon（IFN -）-γ in smoke-induced chronic obstructive pulmonary disease （COPD） in rats. Methods Rat model of COPD was established by exposure to cigarette smoke daffy for 75 days. Total and differential cell counts in bronchoalveolar lavage fluid （BALF） were measured. Levels of IL-9 and IFN-γ in serum, BALF and lung tissue were measured by ELISA. Lung tissue sections stained with hemotoxylin and eosin were studied morphologically under light microscope and the mean linear intercept （Lm）, mean alveolar number （MAN） and bronchial wall thickness-to-diameter ratio （TDR） were measured. Results A. Lm and TDR in COPD group were significantly higher than those in the control group while MAN was decreased （ P 〈 0.01 for each）. B. Levels of IL-9 in serum and BALF in the COPD group were significantly higher than those of the control group [ serum： （4.02 ± 2.12） pg/mL vs （1.12 ± 0.73） pg/mL, BALF： （5.73 ± 2.60） pg/mL vs （0.97 ± 0.45） pg/mL, lung tissue： （24.92 ± 6.65）pg/mL vs （11.42 ± 4.23） pg/mL, all P 〈 0.05]. The levels of IFN-γ in serum and lung tissue were significantly lower than those of the control group [serum： （21.54 ± 7.65） pg/mL vs （69.59 ± 13.68）pg/mL, lung tissue： （21.88 ± 6.11）pg/mL vs （69.84 ± 15.35）pg/mL, both P 〈 0.05]. C.Total cell count, maerophage count and neutrophil count in BALF were significantly different between the COPD group and the control group [（5.29± 1.61） ×10^8/L vs （2.10± 1.19） ×10^8/L, （4.42 ± 1.38） ×10^8/L vs （1.84±0.93）×10^8/L, （ 1.32 ± 0.36） ×10^8/L vs （0. 093 ± 0. 043） ×10^8/L, respectively, both P 〈 0.01 ]. D. IL-9 levels in BALF or Lung tissue,but not in serum, were positively correlated with total cell count, macrophage count and neutrophil count in BALF of COPD group （P 〈 0.01 or 0.05）. And there was negative correlation .between the levels of IL-9 and IFN-γ in lung tissu

关 键 词：慢性阻塞性肺疾病 白细胞介素9 Γ干扰素 气道炎症 

分 类 号：R563.9[医药卫生—呼吸系统]