生物材料对人血清中补体C3含量的影响  

Effects of biomaterials on content of complement C3 in human serum

作　　者：黄晢玮[1] 孙皎[1] 顾国珍[1] 钱云芳[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院上海生物材料研究测试中心,上海市200023

出　　处：《中国临床康复》2006年第29期78-80,共3页Chinese Journal of Clinical Rehabilitation

基　　金：上海市重点学科(特色学科)建设项目资助(T0202)~~

摘　　要：目的:观察比较聚乙烯、一次性使用静脉留置针、血浆袋、输血管4种试验材料与人血清接触后,对人补体C3含量的影响。方法:实验于2004-04/2005-02在上海生物材料研究测试中心血液相容性检测室完成。①取健康人新鲜静脉血,获得正常血清。②将0.6cm2聚乙烯、一次性使用静脉留置针、血浆袋、输血管4种试验材料加入0.1mL血清,(37±1)℃水浴中孵育60min。空白血清作为阴性对照材料,橡胶手套作为阳性对照材料。③通过免疫比浊法来测定补体C3的含量的变化。结果:每组材料重复3管。①聚乙烯、一次性使用静脉留置针、血浆袋、输血管4组实验材料接触血清后的补体C3含量均略有减少[依次为(1.049±0.001),(1.066±0.012),(1.053±0.022),(1.100±0.090)g/L]。②4组材料C3浓度值与阴性对照组[(1.075±0.020)g/L]相比差异无显著性(P>0.05)。③阳性对照组则显示材料接触血清后的补体C3含量[(0.350±0.058)g/L]显著低于4组材料及阴性对照组。结论:①聚乙烯、一次性使用静脉留置针、血浆袋、输血管4组实验材料对机体的补体系统均无明显的不良影响。②补体C3含量测定的方法简便,结果明确,可推荐作为生物材料的免疫学评价方法之一。AIM： To study the effects on contents of human complement C3 by contacting four kinds of test materials： polythene, once venous detain, blood plasma sack and race track with human serum. METHODS： The experiment was conducted in the Testing Department of Blood Compatibility, Shanghai Biomaterials Research ＆ Test Center from April 2004 to February 2005. ① Fresh venous blood was drawn from healthy people to obtain normal serum.②0.6 cm2 of polythene, once venous retention needles, blood plasma sack and race track were contacted with 0.1 mL serum to incubate in water for 60 minutes at （37±1）℃. The blank serum were taken as negative control, and a latex glove was taken as the positive control. The immunoturbidimetry was adopted to determine the changes in contents of C3. RESULTS： Materials of each group was repeated for 3 tubes. ①The contents of C3 slightly decreased after contacting with polythene, once venous detention needles, blood plasma sack and race track [which were respectively （ 1.049±0.001 ）, （ 1.066±0.012 ）,（1.053±0.022）, （ 1.100±0.090）g/L]. ②There were no significant differences in concentration of C3 between four material groups and negative control group [（1.075±0.020） g/L], （P〉0.05）. ③ The contents of C3 in complement of positive control group [ （0.350±0.058） g/L] after contacting with serum was obviously reduced than that of four material groups and negative control group. CONCLUSION： ①There are little harmful effects of polythene, once venous detention needles, blood plasma sack and race track on human complement system. ②The measurement of complement C3 is convenient with definite results, which can be recommended as one of the immunological evaluations for biomaterials.

关 键 词：材料试验 补体 散射测浊法和比浊法 

分 类 号：R318[医药卫生—生物医学工程]